Results 131 to 140 of about 1,250,546 (301)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Artificial Bee Colony-Based Optimization for Public Electric Vehicle Charging Station Placement

Intensif: Jurnal Ilmiah Penelitian Teknologi dan Penerapan Sistem Informasi
Background: The urgency of developing Electric Vehicle Charging Stations (EVCS) infrastructure is increasing alongside the need for low-emission mobility and energy efficiency.
Samsurizal Samsurizal, Agung Dwi Cahyo, Arif Nur Afandi, Andi Ahyina Ardina, Resi Kumala Sari   +4 more
doaj   +1 more source

The optimal behaviour of firms facing stochastic costs [PDF]

This paper aims at assessing the optimal behavior of a firm facing stochastic costs of production. In an imperfectly competitive setting, we evaluate to what extent a firm may decide to locate part of its production in other markets different from which ...
Francesco Menoncin, Rosella Nicolini
core  

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Stream Nutrient Load and Concentration Estimation From Minimal Measurements

Geophysical Research Letters
High‐resolution measurements of nutrients in rivers are vital to assess water quality and catchment material balances. Yet, such measurements are often cost‐prohibitive.
Wasif Bin Mamoon, Kun Zhang, Mitul Luhar, Anthony J. Parolari   +3 more
doaj   +1 more source

Optimal Sizing and Locations of Multiple BESSs in Distribution Systems Using Crayfish Optimization Algorithm

IEEE Access
In distribution systems, battery energy storage systems (BESSs) are the key to fully utilize energy generated from all installed distributed generations (DGs). Only one BESS might be inadequate for present distribution systems connected with several DGs.
Krit Wichitkrailat, Suttichai Premrudeepreechacharn, Apirat Siritaratiwat, Sirote Khunkitti   +3 more
doaj   +1 more source

The cooperative regulation of miR‐221 by APE1 and AUF1 impacts p27Kip1 defining a miR signature relevant for cervical cancer

FEBS Open Bio, EarlyView.
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti, Nicolò Gualandi, Gianluca Tell, Giulia Antoniali   +3 more
wiley   +1 more source

Spatial dynamics and convergence: the spatial AK model [PDF]

We study the optimal dynamics of an AK economy where population is uniformly distributed along the unit circle. Locations only differ in initial capital endowments. Despite constant returns to capital, we prove that transition dynamics will set in.
Carmen CAMACHO, Giorgio FABBRI, Raouf BOUCEKKINE   +2 more
core  

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source