Results 141 to 150 of about 52,041 (264)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

An improved sinh cosh optimizer for optimal scheduling of a microgrid with multi-energy resources and storage considering different weather conditions. [PDF]

Sci Rep
Ma J, Shen H, Nowdeh SA.
europepmc   +1 more source

DSF Core: Integrated Decision Support for Optimal Scheduling of Lifetime Extension Strategies for Industrial Equipment. [PDF]

Sensors (Basel), 2023
Kolokas N, Ioannidis D, Tzovaras D.
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Island recovery methods considering optimal scheduling of emergency mobile resources. [PDF]

Sci Rep
Li T, Chen X, Han X, Zhang H.
europepmc   +1 more source

Optimal Scheduling for Laboratory Automation of Life Science Experiments with Time Constraints. [PDF]

SLAS Technol, 2021
Itoh TD, Horinouchi T, Uchida H, Takahashi K, Ozaki H.   +4 more
europepmc   +1 more source

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

FEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey, Vaishnavi Sanjayan, Praveen Singh, Shantanu Sengupta, Anand Kumar Bachhawat   +4 more
wiley   +1 more source

Optimal Scheduling of Microgrids Based on a Two-Population Cooperative Search Mechanism. [PDF]

Biomimetics (Basel)
Wei L, Zhong H.
europepmc   +1 more source

Optimal Scheduling of Campus Microgrid Considering the Electric Vehicle Integration in Smart Grid. [PDF]

Sensors (Basel), 2021
Nasir T, Raza S, Abrar M, Muqeet HA, Jamil H, Qayyum F, Cheikhrouhou O, Alassery F, Hamam H.   +8 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source