Results 121 to 130 of about 3,761,593 (295)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Optimizing Occupancy Sensor Placement in Smart Environments

open access: yesCoRR
Understanding the locations of occupants in a commercial built environment is critical for realizing energy savings by delivering lighting, heating, and cooling only where it is needed. The key to achieving this goal is being able to recognize zone occupancy in real time, without impeding occupants' activities or compromising privacy.
Hao Lu, Richard J. Radke
openaire   +2 more sources

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

OPTIMAL SENSOR PLACEMENT FOR STRUCTURAL HEALTH MONITORING

open access: yes, 2022
Assessment of the quality, functionality and stability of the structures plays vital role throughout its lifetime. To gather information about the status of any structure, Structural Health Monitoring (SHM) has key role.
Pal, Lokesh
core   +1 more source

Optimal sensor placement for artificial swimmers [PDF]

open access: yesJournal of Fluid Mechanics, 2019
Siddhartha Verma   +4 more
openaire   +4 more sources

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

open access: yesFEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain   +11 more
wiley   +1 more source

Damage Identification of Gas Station Double Layer Grid Structure Based on Time Domain Response Sensitivity Analysis

open access: yesBuildings
Gas station canopy grid structures develop local damage during service life, necessitating regular inspection and maintenance to prevent structural collapse.
Yan Wang   +5 more
doaj   +1 more source

Cell surface CD11c as a neutrophil aging marker molecule

open access: yesFEBS Open Bio, EarlyView.
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki   +5 more
wiley   +1 more source

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