Results 191 to 200 of about 3,171,504 (308)

An integrated multi-scale framework for economically feasible landscape connectivity in working agricultural systems. [PDF]

NPJ Sustain Agric
Dsouza KB, Watt GA, Leonenko Y, Moreno-Cruz J.   +3 more
europepmc   +1 more source

Principles of Optimization for an External Thermo-Wall Structure

Optimization for an External Thermo-Walls ere ...
Krasņikovs, Andrejs, Lapsa, Videvuds Ārijs   +1 more
core  

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Ernie-Switcher: RNA foundation model-guided design of programmable RNA devices in a navigable landscape. [PDF]

Bioinformatics
Lei X, Li R, Liu Y, Huang J, Yang Y, Li R, Liu S, Wang P, Jiang H, Xiong W.   +9 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

A Gait-Conditioned Human-in-the-Loop Optimization Framework Based on Gait-Assistance-Response Relationship Model for Exoskeleton Assistance. [PDF]

Bioengineering (Basel)
Han J, Wang L, Ding J, Zhang Y, Liu X, Bian Q, Jing Z, Zhang J.   +7 more
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Bio-Inspired Optimization in Soft Robot Design: Methods, Applications, and Future Directions. [PDF]

Biomimetics (Basel)
Sun Y, Lueth TC.
europepmc   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Automatic single-isocenter multiple-target cranial stereotactic treatment plan optimization via planning system scripting. [PDF]

J Appl Clin Med Phys
Dekker KH, Everaars JA.
europepmc   +1 more source