Results 41 to 50 of about 170,963,246 (207)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Shape Optimization of Free-form Shells Considering Strain Energy and Algebraic Invariants of Parametric Surface [PDF]

, 2009
p. 525-535A new approach is proposed for shape optimization of shell surfaces, where requirements on the aesthetic aspect and the constructability as well as the structural rationality are simultaneously considered in the problem formulation. The surface
FUJITA, Shinnosuke, OHSAKI, Makoto
core  

Considerations of Accuracy and Uncertainty with Kriging Surrogate Models in Single-Objective Electromagnetic Design Optimization

, 2007
The high computational cost of evaluating objective functions in electromagnetic optimal design problems necessitates the use of cost-effective techniques.
Hawe, G., Sykulski, J.K.
core   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Modeling of Curvilinear Steel Rod Structures Based on Minimal Surfaces

, 2021
The article deals with shaping effective curvilinear steel rod roof structures using genetic algorithms by implementing them for the analysis of various case studies in order to find new and efficient structures with positive characteristics.
Jolanta Dzwierzynska, Igor Labuda
core   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Design and Analysis of a Large Casting and Forging Grinding Robot

工程科学与技术
ObjectiveThe demand for automated surface grinding of large castings and forgings continues to increase with production scale and quality requirements. Conventional grinding robot systems, while functional, present significant limitations: large spatial ...
LIU Yi, FENG Zongqiang, MAO Chongbo, HAN Weida, YANG Changan, MA Qifan   +5 more
doaj  

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source

Conformal and asymptotic properties of embedded genus-g minimal surfaces with one end [PDF]

, 2009
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Mathematics, 2009.Includes bibliographical references (p. 79-82).Using the tools developed by Colding and Minicozzi in their study of the structure of embedded minimal surfaces in R3 [12 ...
Bernstein, Jacob, Ph. D. Massachusetts Institute of Technology
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