Results 31 to 40 of about 913 (260)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Persian Learners' Acquisition of Empty Categories in English Null Operator Structures [PDF]
Teaching English Language, 2019 The study investigated the acquisition of empty categories in the English complex infinitival structures. A quasi-experimental design was used in which four groups of Persian learners of English (elementary, lower intermediate, upper intermediate and ...
Vahid Mahmoudi-Gahroue +2 more
doaj
Optionality and Gradience in Persian Phonology: An Optimality Treatment [PDF]
Iranian Journal of Applied Language Studies, 2014 The distribution of the allophones of /?/in certain contexts involves free variation and gradient preferences. An organized survey was conducted to elicit the judgments of 37 native Persian speakers concerning the well-formedness of /?/allophonic ...
Vahid Sadeghi
doaj +1 more source
MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis
FEBS Open Bio, EarlyView.MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss +7 more
wiley +1 more source
Notes on the optionality of agreement
Anuario del Seminario de Filología Vasca "Julio de Urquijo", 1995 -
Antxon Olarrea
doaj +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Empowering Adult Learning How AI Fuels Progress Across Disciplines and Professions
New Directions for Adult and Continuing Education, EarlyView.ABSTRACT This article examines the evolving role of artificial intelligence (AI) as a transformative cognitive partner across educational and professional fields. It explores how AI shifts attention away from routine technical tasks and toward human‐centered skills such as critical thinking, ethical decision‐making, and professional judgment.
Rachel Wlodarsky
wiley +1 more source
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Background and Objectives Multiple sclerosis (MS) exhibits racially disparate rates of disease progression. Black people with MS (B‐PwMS) experience a more severe disease course than non‐Hispanic White people with MS (NHW‐PwMS). Here we investigated structural and functional connectivity as well as structure–function decoupling in the ...
Emilio Cipriano +11 more
wiley +1 more source
Complacency is not an option [PDF]
Blood, 2011 Hematopoietic stem cell transplantation (HSCT) survivors do not have an increased risk for anxiety and depression. Despite the title of a report in this issue of Blood by Sun et al, “Adverse psychological outcomes in long-term survivors of HSCT: A report from the Bone Marrow Transplant ...
openaire +2 more sources