Results 151 to 160 of about 54,050 (267)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Neurons embedded in loop-like motifs act as central hubs for brain-wide integration. [PDF]

J Physiol
Londei F, Arena G, Ferrucci L, Siano F, Marcos E, Ceccarelli F, Aldo G.   +6 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Gait analysis for functional evaluation in a surgical hindlimb suspension model of muscle atrophy. [PDF]

J Physiol
Xu Y, Zhang Z, Chen P, Kellon N, He A, Alizai U, Lee S, Sathish K, Yan Z, Zhou X, Kutz L, Tsilimigras DI, Pawlik TM, Zhu H.   +13 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Microbial diversity in Pyrenean ice caves: extreme cryogenic environments as analogues for extraterrestrial habitability. [PDF]

FEMS Microbes
Muñoz-Hisado V, Puente-Sánchez F, Bartolomé M, Giménez R, Garcia-Lopez E, Moreno A, Cid C.   +6 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

From predisposition to recovery: field evidence of interactions between the gut microbiota and Brachyspira hyodysenteriae infection. [PDF]

Vet Res
Pérez-Pérez L, Arguello H, Cobo-Díaz JF, Galisteo C, Puente H, Gómez-Martínez S, Carvajal A.   +6 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Seed bank contributions and environmental filtering shape seasonal dynamics and restoration potential of submerged macrophytes in Baiyangdian Lake. [PDF]

Front Plant Sci
Mo K, Wang C, Jin Y, Shi Z, Tian Y, Jin L, Liu C.   +6 more
europepmc   +1 more source