Results 61 to 70 of about 4,863,000 (289)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Modifying soil bacterial communities in saline mudflats with organic acids and substrates

Frontiers in Microbiology
AimsThe high salinity of soil, nutrient scarcity, and poor aggregate structure limit the exploitation and utilization of coastal mudflat resources and the sustainable development of saline soil agriculture.
Xiaoyu Liu, Liang Zhong, Ruixue Yang, Huiyan Wang, Xinbao Liu, Wei Xue, He Yang, Yixin Shen, Jianlong Li, Jianlong Li, Zhengguo Sun, Zhengguo Sun   +11 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

GROWING MEDIA FOR ORGANIC TOMATO PLANTLET PRODUCTION [PDF]

, 2001
The choice of growing media and the strategy of application of organic fertilizers is considered one of the largest challenges for production of organic tomato plantlets for transplanting.
Nielsen, Kai Lønne, Thorup-Kristensen, Kristian   +1 more
core   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

The effect of organic acids as leaching agents for hydrometallurgical recovery of metals from PCBs

Metalurgija, 2022
The hydrometallurgical treatment, compared to other recycling processes, is of great interest due to its higher efficiency and better economy. In hydrometallurgy, popular agents used in the leaching processes of e-waste include inorganic acids, ammonia ...
M. Lisinska, B. Gajda, M. Saternus, S. Brozová, T. Wojtal, M. Rzelewska-Piekut   +5 more
doaj  

Research Program for Organic Food and Farming in Finland [PDF]

, 2005
The Finnish Ministry of Agriculture and Forestry started a three-year Research Programme on Organic Food and Farming in 2003. The programme consists of 15 projects on different themes covering the whole food chain and has an annual budget of about 2.2 ...
Nykaenen, Arja, Nykänen, Arja
core  

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Binding of metals to macromolecular organic acids in natural waters [PDF]

, 2008
Trace metal speciation and bioavailability have become keys to current day toxicity and risk assessments. For many metals, macromolecular organic acids constitute the major ligand in fresh water and soil solution.
van Schaik, Joris W. J.
core   +1 more source