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Orthopoxvirus Purification for High Containment Laboratories
Purification of large viruses in a high containment laboratory can create unique challenges. Traditional purification methods for large viruses rely on equipment and techniques that are not ideal for high containment work. Poxvirus purification has long relied on the use of Genetron®, a reagent that is no longer available.Laura, Hughes +4 more
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Mouse Models for Studying Orthopoxvirus Respiratory Infections
2004Concern regarding the use of variola and monkeypox viruses as bioterrorist agents has led to an increased study of orthopoxviruses to understand the molecular and cellular basis of pathogenesis and develop safe and effective antivirals and vaccines against smallpox.
Jill, Schriewer +2 more
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Inactivation of orthopoxvirus for diagnostic PCR analysis
Journal of Virological Methods, 2007Diagnoses of ongoing viral infections commonly rely on PCR methodology. Sample material that may contain hazardous virus should be efficiently inactivated in biological containment or bed-side before diagnostic PCR analysis. Surprisingly little documentation is available for inactivation of human viral pathogens by inactivation reagents that allow for ...
Lasse, Vinner, Anders, Fomsgaard
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The mechanism of cytoplasmic orthopoxvirus DNA replication
Cell, 1981Orthopoxvirus DNA replication occurs in the cytoplasm of infected cells within discrete foci designated as virosomes. We show that newly synthesized rabbit poxvirus (RPV) virosomal DNA consists predominantly of concatamers wherein unit length molecules are joined by fusion of two left (LL) or right (RR) ends, resulting in genomes aligned in alternating
R W, Moyer, R L, Graves
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Two types of deletions in orthopoxvirus genomes
Virus Genes, 1995The genome nucleotide sequences of two strains of variola major virus and one strain of vaccinia virus were compared. One hundred and sixty-eight short (less than 100 bp in length) and eight long (more than 900 bp in length) deletions, four deletion/insertion regions, and four regions of multiple mutational differences between variola and vaccinia ...
S N, Shchelkunov, A V, Totmenin
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Genus Orthopoxvirus: Variola virus
2007Variola major virus caused the human disease smallpox; interpretations of the historic record indicate that the initial introduction of disease in a naive population had profound effects on its demographics. Smallpox was declared eradicated by the World Health Organization (WHO) in 1980.
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Orthopoxvirus Genome Sequencing, Assembly, and Analysis
Poxviruses have exceptionally large genomes compared to most other viruses, which represent unique challenges to sequencing and assembly due to complex features such as repeat elements and low complexity sequences. The 2022 global mpox outbreak led to an unprecedented level of poxvirus sequencing as public health and research institutions faced with ...Crystal M, Gigante +2 more
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Genus Orthopoxvirus: Vaccinia virus
2007Vaccinia virus (VACV) and Cowpox virus (CPXV) have played seminal roles in human medical and biological science. In 1796 Jenner used CPXV as the first human vaccine and, subsequently, widespread immunization with the related orthopoxvirus (OPV), VACV, led to the eradication of smallpox in 1980.
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Mécanismes d'échappement immunitaire des Orthopoxvirus Orthopoxvirus immune evasion mechanism
Médecine et Maladies Infectieuses, 2004F Messud-Petit, S Bertagnoli
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