SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Exploring techniques to distinguish between real images and those generated using stable diffusion XL. [PDF]
Sanders B, Morrison D, Harris-Birtill D.
europepmc +1 more source
Naked cuticle is essential for Drosophila wing development beyond Wingless signaling
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley +1 more source
GelInsight: Open-source software for large-sample DNA fragmentation quality control in gel electrophoresis images. [PDF]
Bautista KJB +4 more
europepmc +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
Enhancing healthcare outcome with scalable processing and predictive analytics via cloud healthcare API. [PDF]
Salehi SS +7 more
europepmc +1 more source
Bridging Technology and Healthcare: A Bibliometric Review of Assistive Technologies in Hospital Environments. [PDF]
Roy D, Gkiolnta E, Fragulis GF.
europepmc +1 more source
An efficient framework for scheduling security-critical tasks in resource-limited mobile edge computing using hybridized gold rush with golden jackal optimization strategy. [PDF]
Vhatkar K +7 more
europepmc +1 more source
Triboostcardio ensemble model for cardiovascular disease detection using advanced blockchain-enabled health monitoring. [PDF]
Mayuranathan M +5 more
europepmc +1 more source
Full-parameter-modulated three-dimensional vectorial generalized vortex array. [PDF]
Zhang X +11 more
europepmc +1 more source

