Results 61 to 70 of about 1,868,350 (238)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Association Between Experimental Pain Thresholds and Trajectories of Postoperative Recovery Measures After Benign Hysterectomy

Journal of Pain Research, 2022
Peter Lukas,1 Björn Gerdle,2 Lena Nilsson,3 Ninnie Borendal Wodlin,1 Mats Fredrikson,4,5 Lars Arendt-Nielsen,6,7 Preben Kjølhede1 1Department of Obstetrics and Gynecology in Linköping, and Department of Biomedical and Clinical Sciences, Faculty of ...
Lukas P, Gerdle B, Nilsson L, Wodlin NB, Fredrikson M, Arendt-Nielsen L, Kjølhede P   +6 more
doaj  

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Value-Added Roles of Medical Students During the COVID-19 Pandemic: Assessment of Medical Students’ Perceptions and Willingness in Sri Lanka

International Journal of General Medicine, 2021
Nuwan Darshana Wickramasinghe,1 Shamalee Wasana Jayarathne,2 Senaka Devendra Pilapitiya3 1Department of Community Medicine, Faculty of Medicine and Allied Sciences, Rajarata University of Sri Lanka, Saliyapura, 50008, Sri Lanka; 2Medical Education Unit ...
Wickramasinghe ND, Jayarathne SW, Pilapitiya SD   +2 more
doaj  

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Community Level of COVID-19 Information Exposure and Influencing Factors in Northwest Ethiopia

Risk Management and Healthcare Policy, 2020
Animut Tagele Tamiru,1 Bayew Kelkay Rade,1 Eden Bishaw Taye,2 Zelalem Nigussie Azene,3 Mehari Woldemariam Merid,4 Atalay Goshu Muluneh,4 Getahun Molla Kassa,4 Melaku Kindie Yenit,4 Asefa Adimasu Taddese,4 Kassahum Alemu Gelaye,4 Demiss Mulatu Geberu,5 ...
Tamiru AT, Rade BK, Taye EB, Azene ZN, Merid MW, Muluneh AG, Molla GM, Yenit MK, Taddese AA, Gelaye KA, Geberu DM, Tilahun SY, Mekonnen HS, Azagew AW, Wubneh CA, Belay GM, Assimamaw NT, Agegnehu CD, Azale T, Andualem Z, Dagne H, Gashaye KT, Kabito GG, Mekonnen TH, Daba S, Azanaw J, Adane T, Alemayehu M   +27 more
doaj  

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source