Results 191 to 200 of about 1,574,831 (273)

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Using the readiness assessment for pragmatic trials (RAPT) model to structure partner engagement and strengthen pragmatic research readiness. [PDF]

J Clin Transl Sci
Baier RR, Serina PT, Reddy A, Stanislawski A, Hunkins N, McCreedy E.   +5 more
europepmc   +1 more source

Assessing the Evidence for Context-Sensitive Effectiveness and Safety of Patient Safety Practices: Developing Criteria (Prepared under Contract No. HHSA-290-2009-10001C). [PDF]

, 2010
and the PSP Technical Expert Panel, Dy, S, Foy, RC, Hempel, S, McDonald, K, Ovretveit, J, Pronovost, PJ, Rubenstein, L, Shekelle, PG, Taylor, SL, Wachter, RM   +10 more
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

From Knowledge Graphs to Digital Twins: Perspectives on Modeling Patient Outcomes for Health Care Quality Assessment.

J Med Internet Res
Nitschke AK, Diaz Ochoa JG, Neumaier S, Knott M.   +3 more
europepmc   +1 more source

Integrating Clinical, Functional, and Patient-Reported Outcomes in Haemophilia Care: A Delphi-Based Consensus on a New Monitoring Tool. [PDF]

J Clin Med
Molinari AC, Baldacci E, Barillari G, Coluccia A, Coppola A, Giuffrida AC, Giuffrida G, Gorio C, Linari S, Luciani M, Catini A, Nichele I, Peyvandi F, Pollio B, Tagliaferri A, Valeri F, Villa MR, Zanon E, Napolitano M.   +18 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Evaluating Methodological Coherence and Evidence Recognition in Digital Health Systematic Reviews: Sample-based Meta-research Study. [PDF]

Online J Public Health Inform
Buddrus U, Liebe JD.
europepmc   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Pathing the way from regulatory approval to market access for gene therapy products (GTPs): An integrative review in the US, EU5, Japan and China. [PDF]

Regen Ther
Shi J, Hu H, Ung COL.
europepmc   +1 more source