CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Perhitungan Dan Analisis Nilai Overall Equipment Effectivity (Oee) Pada Cylinder Head Line PT. Toyota Motor Manufacturing Indonesia Jakarta [PDF]
Overall Equipment Effectiveness (OEE) is one of the measuring indicators of machine performance which widely applied by industry actors in the world. Overall Equipment Ejéctiveness (OEE) is a method of measuring the effectiveness of the use of equipment ...
Arifianty, M. S. (Meisarah) +1 more
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Evaluating the cooling rate of hot mix asphalt in tropical climate [PDF]
This paper aims to investigate the environmental effect on cooling rate and to determine the appropriate time available for compaction (TAC) using laboratory tests.
Abdul Hassan, Norhidayah +5 more
core +1 more source
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Implementing total productive maintenance in Nigerian manufacturing industries [PDF]
Remarkable improvements have occurred recently in the maintenance management of physical assets and productive systems, so that less wastages of energy and resources occur.
Eti, Mark C. +2 more
core +1 more source
A Framework for Improving Manufacturing Overall Equipment Effectiveness
Nowadays, manufacturing is getting more and more challenging and complex due to shorter product life cycle, high mixed low volume production situation, low equipment performance and volatile customer demand. To overcome all these detractors and rising demands from all angles, manufacturer needs to be fast and agile, as efficient and as transparent as ...
Kam Choi Ng, Kuan Eng Chong
openaire +2 more sources
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Implementasi Total Productive Maintenance Sebagai Penunjang Produktivitas Dengan Pengukuranoverall Equipment Effectiveness Pada Mesin Rotary Kth-8 (Studi Kasus Pt.Indonesian Tobacco) [PDF]
Produsen tembakau iris dihadapi permasalahan downtime mesin yang besar pada mesin Rotary KTH-8 yang berfungsi sebagai pemotong daun tembakau.Downtime mesin yang besar berdampak pada tingkat produktivitas kegiatan produksi dan jumlah produk yang ...
Efranto, R. Y. (Remba) +2 more
core +1 more source
IMPLEMENTASI PROGRAM ADIWIYATA DALAM MENUMBUHKAN NILAI PROFIL PELAJAR PANCASILA DI SDN PENDEM 01 KOTA BATU [PDF]
Based on the results of observations and interviews, SDN Pendem 1 Batu City was an Adiwiyata school in 2015 and has Adiwiyata activities which can foster Pancasila student profile character values in students.
Safitri, Amita
core

