Results 61 to 70 of about 548,440 (263)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Studies of the Oxygenated Compound of Cytochrome Oxidase

Journal of Biological Chemistry, 1968
Abstract Results of rapid reaction experiments indicate that the first observable product formed after the reaction of oxygen with purified reduced cytochrome oxidase is a spectral form which corresponds to that of the oxidized enzyme. The latter is then slowly converted to "The Compound," or so-called oxygenated form.
D C, Wharton, Q H, Gibson
openaire   +2 more sources

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Influence of Aeration on the Composition of Biocenosis and Efficiency of Removal of Nitrogen-Containing Compounds on Rapid Filters for Drinking Water Treatment

Наукові вісті Національного технічного університету України "Київський політехнічний інститут", 2018
Background. Nitrogen compounds are the most common components of groundwater in Ukraine. Nitrates for humans are not poisonous, but in the human body, they turn into nitrites, affecting the health of the whole organism. One of the possible ways to reduce
Oleksandr V. Kravchenko, Oleksandr Yu. Galkin, Olena S. Panchenko   +2 more
doaj   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source

Oxygen-tolerant Electrochemical CO2 Reduction from Bicarbonate Solutions toward Multicarbon Compounds

Electrochemistry
Electrochemical CO2 reduction to multicarbon products offers a promising pathway for closing the anthropogenic carbon cycle while producing value-added chemicals.
Asato INOUE, Kaito NAGITA, Takashi HARADA, Shuji NAKANISHI, Kazuhide KAMIYA   +4 more
doaj   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Oxygen scavengers as active food packaging: A systematic literature review and network analysis

Food Chemistry Advances
Oxygen scavengers are active packaging materials that extend shelf life and maintain food quality, especially for oxygen-sensitive products. This review examines research trends, innovations, and challenges of oxygen scavengers in packaging from 2000 to ...
Muh Arfan, Andi Dirpan, Adiansyah Syarifuddin, Soumya Majumder   +3 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

FEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento, Danniel Pereira‐Figueiredo, Gabriella Grossi de Lima Dias, Gabriel Ferreira dos Santos, Mariana Rodrigues Pereira, Rafael Brito, Karin C Calaza   +6 more
wiley   +1 more source