SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Deep learning-based multimodal approach for non-invasive prediction and prognostic analysis of immune and angiogenic biomarkers in extrahepatic cholangiocarcinoma. [PDF]
Liu J +6 more
europepmc +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
A radiomics nomogram based on ultrasound for predicting ablation zone disappearance after microwave ablation in patients with papillary thyroid microcarcinoma: A retrospective study. [PDF]
Wen Q +6 more
europepmc +1 more source
Development of P-Y Curves for Monopiles in Clay using Finite Element Model Plaxis 3D Foundation
Dhruba Lal Pradhan
openalex +1 more source
Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe
Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley +1 more source
Plasma Level of Stromal Cell-Derived Factor-1 Provides Insight Into Statin Response, But Not Into Left Ventricular Dysfunction in New Unstable Angina. [PDF]
Murad H, Qutub M.
europepmc +1 more source
Prediction of P-Y Curves for Offshore Monopiles Under Lateral Load Through Machine Learning Method
Ran Yan +3 more
openalex +1 more source
Analysis of risk factors for hypoproteinemia after total hip arthroplasty and construction of a nomogram: A retrospective study. [PDF]
Yu Z, Li Y, Zhang Y, Qiu N, Zhu W.
europepmc +1 more source

