Results 221 to 230 of about 1,911,183 (308)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Publisher Correction: Enhancement of cauliflower (Brassica oleracea var. botrytis) water stress resistance using paclobutrazol and partial root-zone irrigation. [PDF]

Sci Rep
El-Shafie AF, Abdelbaset MM, Youssef EA, Dewedar OM.   +3 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Predictive value of PfEMP1 antibody profiles for the course of controlled human malaria infections. [PDF]

PLoS Pathog
Höppner YD, Krumkamp R, Nagaoka H, Wapler C, Honner H, Turner L, Dejon-Agobé JC, Honkpehedji YJ, Zinsou JF, Held J, Esen M, von Thien H, Bruchhaus I, Sim BKL, Hoffman SL, Kremsner PG, Gilberger TW, Lell B, Tsuboi T, Lavstsen T, Takashima E, Mordmüller B, Bachmann A.   +22 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Solution for the hot spots in adaptive radiotherapy planning for cervical cancer: a case report and review of literature. [PDF]

Front Oncol
Jiang P, Wang X, Cui S, Ren C, Wang Z, Zhang Y, Wu X.   +6 more
europepmc   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source

Assessing circuit function in the developing <i>Xenopus</i> tadpole: a survey of the behavioral toolkit and underlying neural substrates. [PDF]

Front Behav Neurosci
Hayes M, Liu A, Todorovic N, Aizenman CD, He HY.   +4 more
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Temporal trends in chest compression quality during a metronome-guided CPR simulation: a secondary analysis of the RITMICO study. [PDF]

Resusc Plus
Monachino G, Guerini Giusteri V, Cresta R, Currao A, Baldi E, Savastano S, Cortegiani A, Ippolito M, Gargano A, Metelmann C, Metelmann B, Hölzing CR, Ganter J, Mueller M, Benvenuti C, Tomola S, Pinetti P, Ingrassia PL, Conte G, Faraci FD, Caputo ML.   +20 more
europepmc   +1 more source