Results 51 to 60 of about 512,921 (340)

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Electrophoresis : what does a century old technology hold for the future of separation science? [PDF]

, 2013
Electrophoretic separation was first demonstrated in the year of 1807 and has since been a staple tool used by biologists and chemists for more than a century since its inception.
Chin, Jason Kee Chian, Kah, Hay Yuen, Sieo, Chin Chin, Yiap, Beow Chin   +3 more
core  

Chemical analysis and aqueous solution properties of Charged Amphiphilic Block Copolymers PBA-b-PAA synthesized by MADIX [PDF]

, 2007
We have linked the structural and dynamic properties in aqueous solution of amphiphilic charged diblock copolymers poly(butyl acrylate)-b-poly(acrylic acid), PBA-b-PAA, synthesized by controlled radical polymerization, with the physico-chemical ...
Berret, J. -F., Cottet, H., Futterer, T., Jacquin, M., Muller, P., Talingting-Pabalan, R., Theodoly, O.   +6 more
core   +5 more sources

The C‐terminal region of KIF26B is indispensable for nephron progenitor condensation and kidney formation in mice

FEBS Open Bio, EarlyView.
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura, Kengo Furuichi, Hiromitsu Akamatsu, Naoki Yamamoto, Sako Keisuke, Akihiko Koshino, Taichiro Minami, Megumi Oshima, Akinori Hara, Norihiko Sakai, Miho Shimizu, Kenta Takahashi, Minako Yamamura, Shin‐ichi Horike, Takiko Daikoku, Kenichi Harada, Yohei Shinmyo, Hiroshi Kawasaki, Takashi Wada, Yasunori Iwata   +19 more
wiley   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Experimental study of particle concentration under electric field

Nihon Kikai Gakkai ronbunshu, 2015
It is difficult to remove well dispersed fine particles from dilute slurry, because filtration and evaporation process requires a lot of time and energy. Electrophoresis is known as a method of moving fine particles.
Shuichi IWATA, Wataru KUBOTA, Masayoshi FUJI, Takashi SHIRAI, Ryo NAGUMO, Hideki MORI   +5 more
doaj   +1 more source

Reporting two hemoglobin J Iran cases, molecular follow-up, or is it insignificant? [PDF]

Caspian Journal of Internal Medicine, 2023
Background: Hemoglobin J is one of the fast hemoglobin that has a more negative charge due to β77His→Asp substitution. Acquisition of this hemoglobin is not associated with any specific clinical sign, but the combination of this hemoglobinopathy with ...
Amin Solouki, Tahereh Manoochehrabadi, Farzaneh Korani, Mohammadreza Farshchi   +3 more
doaj  

Electroblotting onto activated glass. High efficiency preparation of proteins from analytical sodium dodecyl sulfate-polyacrylamide gels for direct sequence analysis [PDF]

, 1986
We have developed a new method for the isolation of proteins for microsequencing. It consists of electrophoretic transfer (electroblotting) of proteins or their cleavage fragments onto activated glass filter paper sheets immediately after separation by ...
Aebersold, Ruedi H., Hood, Leroy E., Kent, Stephen B. H., Teplow, David B.   +3 more
core  

Brownian Dynamics Studies on DNA Gel Electrophoresis. I. Numerical Method and Quasi-Periodic Behavior of Elongation-Contraction Motions

, 2001
Dynamics of individual DNA undergoing constant field gel electrophoresis (CFGE) is studied by a Brownian dynamics (BD) simulation method which we have developed.
Azuma, Ryuzo, Takayama, Hajime
core   +1 more source

Shaping Ti3C2 MXene Nanospheres for Precision Near‐Infrared Photothermal Therapy

Advanced Functional Materials, EarlyView.
In this study, we report producing spherical MXenes via fs laser fragmentation of Ti3C2 flakes in liquid medium. The nanoparticles demonstrated pronounced light absorption and high photothermal conversion efficiencies of 68% and 63% under heating with NIR‐I and NIR‐II lasers, respectively.
Julia S. Babkova, Ivan V. Zelepukin, Konstantin G. Shevchenko, Gleb V. Tikhonowski, Lyubov V. Gorelik, Anton A. Popov, Anna S. Sogomonyan, Alina Yu. Kapitannikova, Ivan S. Kazantsev, Dmitry V. Dyubo, Daria A. Panova, Daniil I. Tselikov, Anton A. Minnekhanov, Aziz B. Mirkasymov, Georgy A. Ermolaev, Alexander V. Syuy, Gleb I. Tselikov, Aleksey V. Arsenin, Andrei V. Kabashin, Kostya S. Novoselov, Sergey M. Deyev, Valentyn S. Volkov   +21 more
wiley   +1 more source