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Cutting Sections of Paraffin-Embedded Tissues
Cold Spring Harbor Protocols, 2008INTRODUCTIONThis protocol describes the sectioning of tissues embedded in paraffin blocks. Paraffin sections require extensive fixation and processing steps but provide superior morphology compared with other sectioning methods. Sectioning paraffin blocks requires experience and should be learned from an experienced researcher, if possible.
Fischer, Andrew H. +3 more
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Paraffin Embedding Tissue Samples for Sectioning
Cold Spring Harbor Protocols, 2008INTRODUCTIONThis protocol describes a method for embedding tissues in paraffin blocks for sectioning. Paraffin sections require extensive fixation and processing steps, but provide superior morphology compared with other sectioning methods.
Fischer, Andrew H. +3 more
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A Disposable Plastic Box for Paraffin Embedding
Stain Technology, 1958A sheet of cellulose acetate about 0.01 inch thick is clamped over a mold, heated to softness by an electric heater and drawn down over the mold by means of a vacuum. When cooled, the sheet, now formed into embedding boxes, is removed from the clamp. Boxes so made are inexpensive enough to be disposable but can be reused, since the sides of the boxes ...
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Softening Paraffin-Embedded Plant Tissues
Stain Technology, 1953Soaking paraffin-embedded plant specimens 2–3 days at 37°C. in a mixture of glycerol, 10 ml., Dreft, 1 g., and water 90 ml. is an effective means of softening them prior to sectioning. One side of the paraffin block must be pared away to expose the tissue before immersion in the softening solution.
S M, ALCORN, P A, ARK
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Multiparameter Immunofluorescence on Paraffin-Embedded Tissue Sections
Applied Immunohistochemistry & Molecular Morphology, 2006Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations.
Frauke, Bataille +7 more
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Orienting and Embedding Small Objects in Paraffin
Stain Technology, 1956A beaker filled with ice cubes and water is covered with a plate of sheet metal. Over this is placed a paper embedding boat full of molten paraffin, and an inch or two above it is held a 60-watt soldering iron. The height of the soldering iron is then adjusted so that the paraffin at the lower half of the embedding dish becomes semisolid and the upper ...
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An Improved Embedding Box for Paraffin
Stain Technology, 1950(1950). An Improved Embedding Box for Paraffin. Stain Technology: Vol. 25, No. 2, pp. 113-113.
E. Russell Hayes, Nancy P. Hayes
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Staining Paraffin Embedded Sections of Scald of Barley before Paraffin Removal
Biotechnic & Histochemistry, 1997Staining of paraffin embedded sections with periodic acid-Schiff reagent and fast green before paraffin removal resulted in differentiation of barley seed and leaf tissue from fungal structures of Rhynchosporium secalis. Crystal violet, toluidine blue O and antiline blue also successfully stained fungal structures of R.
K, Xi, P A, Burnett
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2007
1. Nakon prethodne fiksacije i dehidracije tkivo se obično nalazi u tzv. intermediju. Najčešće se u tu svrhu koristi ksilol. Tkivo se mora prožeti intermedijem sve dok ne padne na dno posude odnosno ne postane prozirno3. 2. Uzorak tkiva prebaciti u posudicu s mješavinom ksilola i parafina (1:1) (termostat, 56° C 10 min.)4. 3. Tkivo staviti u posudicu s
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1. Nakon prethodne fiksacije i dehidracije tkivo se obično nalazi u tzv. intermediju. Najčešće se u tu svrhu koristi ksilol. Tkivo se mora prožeti intermedijem sve dok ne padne na dno posude odnosno ne postane prozirno3. 2. Uzorak tkiva prebaciti u posudicu s mješavinom ksilola i parafina (1:1) (termostat, 56° C 10 min.)4. 3. Tkivo staviti u posudicu s
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Embedding Paraffins for Eye Microtomy
Journal of Histotechnology, 1977Abstract Embedding paraffins commonly used for eye microtomy are discussed. A relatively new paraffin product may be used also for eye microtomy and light microscopy preparations of excellent quality.
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