Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples [PDF]
, 2016 Fragmented RNA from formalin-fixed paraffin-embedded (FFPE) tissue is a known obstacle to gene expression analysis. In this study, the impact of RNA integrity, gene-specific reverse transcription and targeted cDNA preamplification was quantified in terms
Cuvelier, Claude +5 more
core +2 more sources
A novel method for RNA extraction from FFPE samples reveals significant differences in biomarker expression between orthotopic and subcutaneous pancreatic cancer patient-derived xenografts. [PDF]
, 2016 Next-generation sequencing (NGS) can identify and validate new biomarkers of cancer onset, progression and therapy resistance. Substantial archives of formalin-fixed, paraffin-embedded (FFPE) cancer samples from patients represent a rich resource for ...
Adamian, Yvess +12 more
core +2 more sources
Sentinel lymph node biopsy in rats. Comparison between paraffin and frozen section analysis [PDF]
Acta Cirúrgica Brasileira, 2014 PURPOSE: To simulate a lymph node metastasis in an animal model using activated carbon, assess their identification in frozen section analysis and compare with histopathological examination in paraffin.
Ivan Dunshee de Abranches Oliveira Santos Filho +5 more
doaj +1 more source
Comparison of Varying Tissue Freezing Methods on Murine Colonic Tissue [PDF]
, 2018 Histology often requires a tissue specimen to be embedded so that it may be sectioned, stained, and mounted on a microscope slide for viewing. One common method of tissue embedding for rapid histology is freezing, since freezing allows tissue to be ...
Hughes, James
core +2 more sources
Improved arteriogenesis with simultaneous skeletal muscle repair in ischemic tissue by SCL plus multipotent adult progenitor cell clones from peripheral blood [PDF]
, 2004 Background: The CD34- murine stem cell line RM26 cloned from peripheral blood mononuclear cells has been shown to generate hematopoietic progeny in lethally irradiated animals.
Gottgens, Berthold +7 more
core +1 more source
Pediatric Blood &Cancer, EarlyView.ABSTRACT Claudin‐6 has emerged as a promising immunotherapeutic target, yet protein‐level data in atypical teratoid/rhabdoid tumors (AT/RTs) have been inconsistent. We analyzed 36 well‐characterized AT/RT samples and found membranous claudin‐6 protein expression in 58% of cases, with striking enrichment in the molecular subgroup AT/RT‐TYR (100%) and ...
Victoria E. Fincke +4 more
wiley +1 more source
A case of dirofilaria in human eye localization
Microbiologia Medica, 2010 We describe a case of infestation by Dirofilaria repens in an immunocompetent female who goes to the emergency room for a feeling irritable left eye.
Cristina Lugli +2 more
doaj +1 more source
Degeneration of postovulatory follicles in the Iberian sardine Sardina pilchardus: structural changes and factors affecting resorption [PDF]
, 2007 Inaccuracy in the aging of postovulatory follicles (POFs) and in estimating the effect of temperature on the resorption rate of POFs may introduce bias in the determination of the daily spawning age classes with the daily egg production method (DEPM). To
Ganias , Konstantinas +2 more
core
The miR-139-5p regulates proliferation of supratentorial paediatric low-grade gliomas by targeting the PI3K/AKT/mTORC1 signalling [PDF]
, 2018 Paediatric low-grade gliomas (pLGGs) are a heterogeneous group of brain tumours associated with a high overall survival: however, they are prone to recur and supratentorial lesions are difficult to resect, being associated with high percentage of disease
Antonelli, Manila +19 more
core +1 more source
The Paraffin-Embedded RNA Metric (PERM) for RNA Isolated from Formalin-Fixed, Paraffin-Embedded Tissue [PDF]
BioTechniques, 2016 RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue is commonly evaluated in both investigative and diagnostic pathology. However, the quality of the data is directly impacted by RNA quality. The RNA integrity number (RIN), an algorithm based on a combination of electrophoretic features, is widely applied to RNA isolated from paraffin ...
Joon-Yong Chung +2 more
openaire +4 more sources