Results 41 to 50 of about 109,391 (304)

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Paraffin Embedding and Histological Analyses of Sw71-Spheroids as Human Blastocyst-like Surrogates

Organoids
Implantation studies are extremely important to solve reproductive problems since about 60% of abortions occur around this period. The 3D in vitro models emerge as closest to the in vivo structures and processes.
Marina Alexandrova, Mariela Ivanova, Martina Metodieva, Antonia Terzieva, Tanya Dimova   +4 more
doaj   +1 more source

An inexpensive alternative equipment for the plant material embedding in the paraffin under the vacuum

Brazilian Archives of Biology and Technology, 2008
The present work describes an equipment constructed using inexpensive material for embedding the plant material in the paraffin under the vacuum, using an oven and a vacuum pump.
Carlos André Espolador Leitão, Angelo Luiz Cortelazzo   +1 more
doaj   +1 more source

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

Molecular Oncology, EarlyView.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice, Jose I. Lopez, Aitziber Ugalde‐Olano, Maite Zufiaurre, Ianire Astobiza, Natalia Martin‐Martin, Laura Bozal‐Basterra, Saioa Garcia‐Longarte, Amaia Zabala‐Letona, Sofia Rey, Aida Santos‐Martin, Miguel Unda, Ana Loizaga‐Iriarte, Mariona Graupera, Paolo Nuciforo, Arkaitz Carracedo, Isabel Mendizabal   +16 more
wiley   +1 more source

Can intraoperative ultrasound replace the frozen section in the assessment of ex vivo sentinel lymph node biopsy in early breast cancer in countries with limited resources?

The Egyptian Journal of Radiology and Nuclear Medicine, 2021
Background Accurate staging and proper management of axillary lymph nodes (ALNs) in breast cancer patients are important for treatment. Surgical management of the axilla has evolved greatly in the last 20 years.
Rasha Wessam Abdel Rahman, Emad Salaheldin Khallaf, Lamia Adel Salaheldin, Mohamed Nasr Hafez, Mohannad Aly Fayed, Somia Abdulatif Mahmoud Soliman   +5 more
doaj   +1 more source

Non-specific binding of antibodies in immunohistochemistry: Fakes and facts [PDF]

, 2011
Protocols for blocking non-specific antibody (Ab) binding in immunohistochemistry are based on rather contradictory and outdated reports. This prompted us to prove, whether non-specific Ab binding may really lead to unwanted background staining in ...
Igor Buchwalow, Markus Tiemann, Vera Samoilova, Werner Boecker   +3 more
core   +1 more source

Expression and Functional Studies on the Noncoding RNA, PRINS. [PDF]

, 2012
PRINS, a noncoding RNA identified earlier by our research group, contributes to psoriasis susceptibility and cellular stress response. We have now studied the cellular and histological distribution of PRINS by using in situ hybridization and demonstrated
Amin, Anikó Göblös, Attila Dobozy, Bata-Csorgo, Bata-Csorgo, Belso, Beylot-Barry, Borer, Bos, Bos, Bowcock, Bowcock, Carlen, Di Carlo, Duffy, Feuerstein, Feuerstein, Feuerstein, Feuerstein, Hernandez-Verdun, István Németh, Itahana, Krisztina Szegedi, Krueger, Kurki, Lajos Kemény, Lebwohl, Lee, Lindstrom, Livak, Mattick, Moll, Mária Antal, Márta Széll, Nomura, Palaniswamy, Papadopoulou, Peter, Pivarcsi, Pivarcsi, Polyanka, Rinn, Sarolta Bacsa, Sonkoly, Spector, Stoeckman, Szegedi, Szell, Szymanski, Takemura, Tarapore, Tiedge, Ting, Van Hooser, Wang, Wu, Yogev, Yusufzai, Zalfa, Zaric, Zhou, Zsuzsanna Bata-Csörgő   +61 more
core   +2 more sources

PARP inhibition and pharmacological ascorbate demonstrate synergy in castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
Pharmacologic ascorbate (vitamin C) increases ROS, disrupts cellular metabolism, and induces DNA damage in CRPC cells. These effects sensitize tumors to PARP inhibition, producing synergistic growth suppression with olaparib in vitro and significantly delayed tumor progression in vivo. Pyruvate rescue confirms ROS‐dependent activity.
Nicolas Gordon, Peter T. Gallagher, Orly I. Richter, Neermala Poudel Neupane, Amy C. Mandigo, Jennifer J. McCann, Emanuela Dylgjeri, Irina Vasilevskaya, Christopher McNair, Channing J. Paller, Wm. Kevin Kelly, Karen E. Knudsen, Matthew J. Schiewer, Ayesha A. Shafi   +13 more
wiley   +1 more source

Multicolor Histochemical Staining for Identification of Mineralized and Non-Mineralized Musculoskeletal Tissue: Immunohistochemical and Radiological Validation in Decalcified Bone Samples

Bioengineering, 2022
Histochemical staining of paraffin-embedded decalcified bone samples is commonly used in preclinical research of musculoskeletal diseases, enabling the visualization of multiple tissue components by the application of chromogens.
Yu Sun, Heike Helmholz, Regine Willumeit-Römer   +2 more
doaj   +1 more source

Gene expression analysis in specific cell populations of bovine blastocysts using laser capture microdissection [PDF]

, 2012
Laser capture microdissection (LCM) has become a powerful technique for the isolation of specific cell populations from heterogeneous tissues for gene expression analysis.
Burvenich, Christian, Cornillie, Pieter, De Spiegelaere, Ward, De Spiegeleer, Bart, Goossens, Karen, Peelman, Luc, Stevens, Mieke, Van Soom, Ann   +7 more
core