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Microtomy: Cutting Formalin-Fixed, Paraffin-Embedded Sections
Methods in molecular biology, 2018Formalin-fixed, paraffin-embedded tissue (FFPE) still plays an important role in biobanking, since it is comparatively easy to obtain and store in comparison to fresh frozen tissue. They are stored as paraffin or FFPE blocks. Unstained slides derived from FFPE blocks may be used for hematoxylin and eosin histology, special stains, immunohistochemistry,
Joanne, Sy, Lee-Cyn, Ang
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Preparing Paraffin Tissue Sections for Staining
Cold Spring Harbor Protocols, 2021Most histological studies are performed on formalin-fixed, paraffin-embedded (FFPE) tissue samples. Therefore, there is an extensive atlas of most tissues and organs prepared from these sources, and comparing the location of antigens to these data is immediately informative.
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Postadhesive Technique for Archival Paraffin Sections
Biotechnic & Histochemistry, 1997We present a postadhesive protocol for adhering paraffin sections of archival material to microscope slides. Appropriately posttreated sections, subsequently processed for immunohistochemistry, remained attached to the slides and were well preserved with no signs of artifacts, such as scratching and shrinkage.
M, Pateraki, G, Kontogeorgos
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Acridine Orange Fluorescence in Paraffin Sections
Stain Technology, 1962The technique of staining with acridine orange for fluorescence microscopy of fresh animal and plant cells, chiefly for the detection of ribonucleic acid in the cytoplasm, was brought to a high degree of perfection by Schummelfeder (1950) and has been developed further by Bertalanffy and Bickis (1956).
H A, ABRAMS, H, ELIAS
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Cutting Sections of Paraffin-Embedded Tissues
Cold Spring Harbor Protocols, 2008INTRODUCTIONThis protocol describes the sectioning of tissues embedded in paraffin blocks. Paraffin sections require extensive fixation and processing steps but provide superior morphology compared with other sectioning methods. Sectioning paraffin blocks requires experience and should be learned from an experienced researcher, if possible.
Fischer, Andrew H. +3 more
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Paraffin Embedding Tissue Samples for Sectioning
Cold Spring Harbor Protocols, 2008INTRODUCTIONThis protocol describes a method for embedding tissues in paraffin blocks for sectioning. Paraffin sections require extensive fixation and processing steps, but provide superior morphology compared with other sectioning methods.
Fischer, Andrew H. +3 more
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Remnants of paraffin (“Paraffin-Inclusions”) in histological sections
Virchows Archiv für Pathologische Anatomie und Physiologie und für Klinische Medizin, 1961The birefringent deposits in lymphocytes with “atypical nuclei”, as described byE. H. Tompkins, represent remnants of undissolved paraffin (“paraffin-inclusions”).
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Multiparameter Immunofluorescence on Paraffin-Embedded Tissue Sections
Applied Immunohistochemistry & Molecular Morphology, 2006Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations.
Frauke, Bataille +7 more
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Paraffin Sections of Tissue Fragments
Stain Technology, 1947Pathologists are frequently called upon to examine minute fragments of tissue obtained by aspiration or other similar means. Although the smear technic is generally satisfactory for this purpose, there are occasions when sections are preferable. This is the case when study of the interrelationship of cells is desired, or when one wishes to avoid the ...
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