Results 121 to 130 of about 524,430 (317)
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Faster parameter estimation using risk-sensitive filters
, 1998 In this paper, we propose a risk-sensitive approach to parameter estimation for hidden Markov models (HMMs). The parameter estimation approach considered exploits estimation of various functions of the state, based on model estimates.
Athuraliya, Sanjeewa +3 more
core +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Estimation of the long memory parameter in non stationary models: A Simulation Study [PDF]
In this paper we perform a Monte Carlo study based on three well-known semiparametric estimates for the long memory fractional parameter. We study the efficiency of Geweke and Porter-Hudak, Gaussian semiparametric and wavelet Ordinary Least-Square ...
Mohamed Boutahar, Rabeh Khalfaoui2
core
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Comparison of estimation limits for quantum two-parameter estimation
Physical Review ResearchMeasurement estimation bounds for local quantum multiparameter estimation, which provide lower bounds on possible measurement uncertainties, have so far been formulated in two ways: by extending the classical Cramér-Rao bound (e.g., the quantum Cramér ...
Simon K. Yung +4 more
doaj +1 more source
Remarks on the estimation of position parameters
Statistics in Transition new series, 2017 The article contains some theoretical remarks about selected models of position parameters estimation as well as numerical examples of the problem. We ask a question concerning the existence of possible measures of the quality of interval estimation and we mention some popular measures applied to the task.
openaire +3 more sources
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
FEBS Open Bio, EarlyView.Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Comparative Studies of Methods for Accurate Hurst Parameter Estimation
Over the last decade the traffic of the packet-based networks shows self-similar or long-range dependent nature. Such behaviour raises issues of accuracy in resource allocation and related guarantee required of QoS parameters for data flow.
Kuļikovs, Mihails +2 more
core