Results 111 to 120 of about 9,078,018 (292)
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
[Objective]The aims of this study are to reveal the characteristics of spatial distribution of soil grain size in Qingtu Lake at the end of Shiyang River, and to provide theoretical basis for ecological restoration in this region. [Methods]Sampling lines
An Fubo +6 more
doaj
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
Due to the high sensitivity of grid-based micro-scale wind–sand flow models to deformation and distortion, this study employs the Smooth Particle Hydrodynamics (SPH) method for numerical simulations.
Wenxiu Gao +3 more
doaj +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
The combined effect of periodic water impoundment and seasonal natural flood events has created a 30 m high water-level fluctuation zone (WLFZ) around the Three Gorges Reservoir (TGR), China, forming a unique eco-landscape.
Wei, J. +5 more
core +1 more source
MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima +8 more
wiley +1 more source
Inferência por redes neurais da distribuição de tamanho de partícula em reações contínuas de polimerização em emulsão [PDF]
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia Química, Florianópolis, 2014As exigências do mercado mundial em relação à produtividade e qualidade dos produtos poliméricos tornam ...
Ferrari, Jarbas Cleber
core
In the paper we present the method of calculating the particle size distribution of the initial granules overgranulated by the “fattening” technology. We have developed a number of different ways for solving the problem for the continuous and periodic ...
Yu. A. Taran +3 more
doaj

