We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt +8 more
wiley +1 more source
A new apparatus for recording particle size distribution
A manometer and sensitive capacitance transducer follow the drop in hydrostatic pressure at a point near the bottom of a column of soil suspension during sedimentation.
Bascomb, C. L.
core +1 more source
Building predictive models of soil particle-size distribution
Is it possible to build predictive models (PMs) of soil particle-size distribution (psd) in a region with complex geology and a young and unstable land-surface? The main objective of this study was to answer this question.
Alessandro Samuel-Rosa +2 more
doaj +1 more source
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
Discrete Element Study of Particle Size Distribution Shape Governing Critical State Behavior of Granular Material [PDF]
Granular soil is a porous medium composed of particles with different sizes and self-similar structures, exhibiting fractal characteristics. It is well established that variations in these fractal properties, such as particle size distribution (PSD ...
Jiang, Mingdong +7 more
core +1 more source
Particle Size and Pore Size Selection on Ordered Mesoporous Silica [PDF]
Controlling both particle size and pore size is critical when using mesoporous silica for fundamental studies. Pore size can be adjusted by modifying the reaction conditions during synthesis, but achieving a narrow particle size selection can be ...
Tianyu, Li
core +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Dependence of shape on particle size for a crushed rock railway ballast [PDF]
Laboratory testing of railway ballast poses practical difficulties because the particle size is often too large for most standard apparatus. There are therefore advantages in developing a scaled material whose behavior is representative of the full size ...
Le Pen, Louis +9 more
core +1 more source
Impact of Particle Size Distribution of Colloidal Particles on Contaminant Transport in Porous Media
The presence of retained colloidal particles causes the retardation of contaminant transport when the contaminant is favorably adsorbed to colloidal particles. Although the particle size distribution affects the retention behavior of colloidal particles,
Jongmuk Won +4 more
doaj +1 more source

