Results 261 to 270 of about 1,217,363 (294)

ABO genotyping by PCR-RFLP and cloning and sequencing

Anthropologischer Anzeiger, 2004
A refined PCR-RFLP based method was established to genotype ABO blood groups. The main objective of this study was to make the techniques also suitable for working with degraded DNA. Specific primer design was carried out to choose fragments shorter than 200 bp as necessary in forensic and archaeological applications.
Haak, W., Burger, J., Alt, K.
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Differentiation of sturgeon species by PCR-RFLP

Food Research International, 1999
Abstract A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNAGlu/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction ...
Philipp Hübner, Jürg Lüthy, Christian Wolf   +2 more
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Comprehensive typing of DQB1 alleles by PCR‐RFLP

Tissue Antigens, 1994
Abstract: The protocols represented in this report can resolve all 22 DQB1 alleles. The second exon of DQB1 was subjected to PCR using two group‐specific primers to obtain DQB1 group 1 (DQ5 and DQ6) and group 2 (DQ2, DQ3, DQ4) specific amplified products, respectively.
Rose Goldstein, Dharmendra P.S. Sengar
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Identification of common species of dermatophytes by PCR-RFLP

Journal of Huazhong University of Science and Technology [Medical Sciences], 2005
To establish a simple, sensitive and effective technique for the identification of six common dermatophytes, polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) targeting Topoisomerase II gene were used. The DNA of 6 common dermatophytes was amplified by primer dPsD1 and then primers dPsD2.
He Ganlin, Li Jiawen, Ding Juan, Tan Zhijan   +3 more
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Automated, PCR-RFLP Genotyping of the Urokinase Gene

Genetic Testing, 2000
The urokinase-type plasminogen activator (u-PA) has been suggested to play a role in the early initiation and progression of atherosclerosis and coronary artery disease (CAD) (Grenett et aL, 1998). Recently, a common genetic polymorphism in the untranslated region of the u-PA gene was shown to be associated with syptomatic CAD.
F. Booyse, Susan M. Sell, F. Blasi
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Identification of clinically relevant yeasts by PCR/RFLP

Journal of Microbiological Methods, 2004
For molecular diagnosis of fungal disease using DNA amplification procedures in the routine laboratory, choice of appropriate target structures and rapid and inexpensive identification of amplification products are important prerequisites. Most diagnostic procedures described thus far are characterized by limited applicability, considerable cost for ...
Thomas Adam, Kurt Possinger, Anja Trost, Orhan Sezer, Barbara Graf, Ulf B. Göbel, Jan Eucker   +6 more
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PCR-RFLP analysis of cpDNA in the genus Abies

Theoretical and Applied Genetics, 1999
We used PCR-RFLP analysis of the chloroplast DNA of the genus Abies (family Pinaceae), to determine if the method could be employed to detect inter-specific variation in this genus and to study how the variation was distributed in different regions of the genome.
Parducci Laura, Szmidt Alfred E
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PCR- RFLP for Identification of Bluetongue Virus Serotypes

Southwestern Entomologist, 2015
Abstract. Bluetongue virus is the etiological agent of a disease characterized by bleeding in domestic and wild ruminants. Different viral strains can infect a ruminant species and severity of infection is variable. Transmission of the virus under natural conditions is through the bite of a mosquito of the genus Culicoides (Ceratopogonidae), but also ...
Rafael González-Alvarez, Oralia Barboza-Quintana, Adriana E. Flores-Suarez, Ángel Lugo-Trampe, Olga Karina Villanueva-Segura, Ivan Delgado-Encizo, Sofía Bernal-Silva, Raquel Garza-Guajardo, Iram P. Rodriguez-Sanchez, Gustavo Ponce-Garcia, Susana Favela-Lara, Laura Elia Martínez-de-Villarreal, Karina del Carmen Trujillo-Murillo   +12 more
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Differentiation of anaerobic polycentric fungi by rDNA PCR-RFLP [PDF]

Folia Microbiologica, 2006
The suitability of restriction fragment length polymorphism (RFLP) analysis of the ribosomal DNA cluster for discriminating two genera of anaerobic polycentric fungi, Orpinomyces and Anaeromyces, was determined. Three PCR-amplified DNA fragments--nuclear small subunit (SSU; 18S rDNA), the nuclear large subunit (LSU; 28S rDNA) and internal transcribed ...
Jakub Mrázek, Kerstin Voigt, K. Fliegerová   +2 more
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Diagnosis of Penicillin Resistance by PCR-RFLP

2003
Streptococcus pneumoniae is an important human pathogen causing a wide spectrum of disease including pneumonia, otitis media, bacteraemia, and meningitis. It is a significant cause of morbidity and mortality worldwide and now penicillin resistance is becoming an ever increasing problem (1-2). Initially, all S.
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