Rapid detection of MDR–Mycobacterium tuberculosis using modified PCR-SSCP from clinical Specimens [PDF]
Asian Pacific Journal of Tropical Biomedicine, 2014 Objective: To design a rapid test to detect the rifampin (RIF) and isoniazid (INH) resistant mutant based on polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique that analyzes the katG, rpoB genes.
Imani Fooladi Abbas Ali +3 more
doaj +4 more sources
Paternal imprinting of the
BMC Genetics, 2004 Background Genomic imprinting is an epigenetic chromosomal modification in the gametes or zygotes that results in a non-random monoallelic expression of specific autosomal genes depending upon their parent of origin.
Krishna Lingegowda +3 more
doaj +2 more sources
Identification of parasitic nematodes by PCR-SSCP of ITS-2 rDNA
Molecular and Cellular Probes, 1997 Fourteen species of parasitic nematodes (order Strongylida) were characterized using a polymerase chain reaction-linked single strand conformation polymorphism technique (PCR-SSCP). The rDNA region spanning the second internal transcribed spacer (ITS-2) was amplified from parasite DNA by PCR.
Robin B Gasser
exaly +3 more sources
3′ Overhangs Influence PCR-SSCP Patterns [PDF]
BioTechniques, 2000 Rachel Forrest +2 more
doaj +3 more sources
Improved Resolution of Asymmetric-PCR SSCP Products [PDF]
BioTechniques, 1997 Richard M. Pokorny +3 more
doaj +4 more sources
Single nucleotide polymorphism rs7908486 of the TCF7L2 gene is highly associated with obesity in the Iraqi population [PDF]
Archives of Biological Sciences, 2021 This study was conducted to assess the possible association between polymorphisms of the transcription factor 7-like 2 (TCF7L2) gene and obese Iraqi adults. DNA was obtained from 158 obese subjects and 142 controls.
Mohammed Amera K. +2 more
doaj +1 more source
Detection and isolation of nuclear haplotypes by PCR‐SSCP [PDF]
Molecular Ecology, 1997 SSCP (single‐strand conformational polymorphism) is used widely in the field of human biomedicine, but its potential as a population genetics tool for the recovery of nuclear gene genealogies remains to be realized. We describe and illustrate a use for SSCP in the physical isolation of nuclear haplotypes that circumvents several difficulties associated
Ortí, G, Hare, MP, Avise, JC
openaire +4 more sources
Genotyping of Salmonella enterica strains from animal and human origin using three molecular techniques [PDF]
Iraqi Journal of Veterinary Sciences, 2022 This study aims to characterize different Salmonella enterica subsp molecularly. enterica strains (n=49) were isolated from human gastrointestinal cases in the Tolima region and poultry from Santander and Tolima regions using PCR-RFLP, PCR-ribotyping ...
Juan S. Cruz-Méndez +2 more
doaj +1 more source
PCR–SSCP: A Method for the Molecular Analysis of Genetic Diseases [PDF]
Molecular Biotechnology, 2007 Single strand conformation polymorphism (SSCP) is a reproducible, rapid and quite simple method for the detection of deletions/insertions/rearrangements in polymerase chain reaction amplified DNA. All the details for the use of PCR-SSCP are presented in the direction of genetic diseases (beta-thalassaemia, cystic fibrosis), optimum gel conditions ...
V Konstantinos, Kakavas +9 more
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Molecular Characteristics of Phytophthora katsurae Using PCR-SSCP Analysis
Research in Plant Disease, 2011 Phytophthora katsurae is the fungus responsible for chestnut ink disease. The objectives of this study were to determine if a single-strand conformation polymorphism (SSCP) analysis of rDNA-ITS region, elongation factor 1 alpha gene and β-tubulin gene ...
Sun Keun Lee +5 more
doaj +1 more source