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PCR-SSCP: A method for detection of mutations
Genetic Analysis: Biomolecular Engineering, 1992PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) analysis is one of the simplest and perhaps one of the most sensitive methods for detection of mutations based on PCR technology. The principles of PCR-SSCP, guidelines for experiments, and applications of this technique in various fields are reviewed.
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Extensive mtDNA diversity in horses revealed by PCR–SSCP analysis
Animal Genetics, 1995SummaryThe hypervariable D‐loop region of mitochondrial DNA (mtDNA) was amplified with the polymerase chain reaction using total horse DNA samples. Analysis of single strand conformation polymorphism (SSCP) of denatured amplification products was carried out by native polyacrylamide (8%) gel electrophoresis followed by silver staining.
S, Marklund +4 more
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Allelic Variation in the Porcine MYF5 Gene Detected by PCR–SSCP
Molecular Biotechnology, 2008The MYF5 gene has been reported to be integral to muscle growth and development, and hence it has been considered as a candidate gene for meat selection programs in pig. To ascertain whether there was variation in the porcine MYF5 gene, we have developed a method of PCR-single-strand conformational polymorphism (PCR-SSCP) analysis.
Sajee, Kunhareang +2 more
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Microhaplotype identified and performed in genetic investigation using PCR-SSCP
Forensic Science International: Genetics, 2017The recently introduced concept of microhaplotype loci has attracted attention in forensics. Previous studies estimated the allele frequencies generally through obtaining genotypic data on the individual SNPs from a larger set of unrelated individuals then phasing microhaplotypes by statistical and computational techniques.
Peng, Chen +11 more
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Polymorphism in the human E-selectin gene detected by PCR-SSCP
Human Genetics, 1994By using the non-isotopic single-strand conformation polymorphism (SSCP) technique to analyse products of the polymerase chain reaction (PCR), we detected a 561-adenine to cytosine substitution resulting in an amino acid exchange from serine to arginine at position 128 of the E-selectin gene.
K, Wenzel, R, Hanke, A, Speer
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No TP53 mutations in neuroblastomas detected by PCR‐SSCP analysis
Genes, Chromosomes and Cancer, 1994AbstractWe have analysed 29 neuroblastomas for TP53 mutations in exons 5 to 8 by means of the polymerase chain reaction in combination with the single‐strand conformation polymorphism technique. We could not detect any mutation. These results indicate that, in contrast to the majority of tumors so far studied, TP53 mutations do not seem to be important
J S, Castresana +6 more
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PCR‐SSCP ANALYSIS OF THE p53 GENE IN TUMOURS OF THE ADRENAL GLAND
Clinical and Experimental Pharmacology and Physiology, 1996SUMMARY1. Mutations of the p53 tumour suppressor gene are relatively common in the aetiology of a wide spectrum of tumour types, both sporadic and familial.2. The majority of mutations of the p53 gene are reported to be in the highly conserved region of exons 5–8.3.
Ballantine, DM +4 more
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Analysis of Microbial Community in Vermifiltration by PCR-SSCP Method
2009 3rd International Conference on Bioinformatics and Biomedical Engineering, 2009Single-strand conformation polymorphism (SSCP) method was performed to investigate the microbial community in vermifiltration. Vermifiltration was a newly ecological and high efficient process to treat sewage and sludge synchronously. The experimental results indicated that PCR-SSCP can more truly reflect the existing system of micro-organisms groups ...
Zhibo Lu +3 more
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Application of PCR-SSCP to Species Identification of Fishery Products
Journal of the Science of Food and Agriculture, 1997A method of DNA analysis has been developed to verify authenticity of labelled raw material of canned fish or in products made from closely related fish species (tuna, eel, salmon, trout and sturgeon). Short segments (123–358 bp) of the mitochondrial cytochrome b gene were amplified by the polymerase chain reaction (PCR) and analysed by single strand ...
Hartmut Rehbein +2 more
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Distribution of MN genotypes detected by PCR-SSCP analysis
International Congress Series, 2003Abstract Genotyping of the MN blood system was performed by means of PCR-single strand conformation polymorphism (PCR-SSCP) analysis. Twelve band patterns corresponding to each MN genotype composed of alleles MG, MT, N1, N2 and NV were detected. In general, MG or N1>MT>N2 in order of decreasing frequency was observed as four common alleles in five ...
N. Nakayashiki +6 more
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