Results 21 to 30 of about 11,641 (223)
Emerging Infectious Diseases, 2001 The use of polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) to study rpoB gene mutations in rifampin-resistant (RIFr) Mycobacterium tuberculosis has yielded contradictory results.
Miriam Bobadilla-del-Valle +8 more
doaj +1 more source
Identification of Myostatin Gene C.960delg Locus Polymorphism in Indonesian Local Sheep by Using Pcr-sscp Method [PDF]
, 2011 Myostatin (MSTN) is a protein inhibit muscle growth. This protein is a member of a superfamily ofmolecules called transforming growth factors beta (TGF-b).
Andreas, E. (E) +4 more
core +1 more source
Polymorphisms in swine candidate genes for meat quality detected by PCR-SSCP
Italian Journal of Animal Science, 2010 In order to identify SNPs in seven candidate genes for meat quality, PCR-SSCP experiments were carried out. Statistical analyses in a population of 240 individuals identified the animals constituting the extreme tails of the Gaussian distribution for ...
Stefania Chessa +4 more
doaj +1 more source
Five patients with biochemical and/or clinical generalized glucocorticoid resistance without alterations in the glucocorticoid receptor gene [PDF]
, 2000 Cortisol resistance (CR) is a rare disease characterized by a generalized reduced sensitivity of end-organs to the actions of glucocorticoids (GCs)
Abs, R. (Elisabeth) +7 more
core +1 more source
Reconstruction of the original mycoflora in pelleted feed by PCR-SSCP and qPCR [PDF]
FEMS Microbiology Letters, 2014 Ground feeds for pigs were investigated for fungal contamination before and after pelleting (subsamples in total n = 24) by cultural and molecular biological methods. A fungal-specific primer pair ITS1/ITS5.8R was used to amplify fungal DNA; PCR products were processed for the PCR-SSCP method. In the resulting acrylamide gel, more than 85% of DNA bands
Samart, Dorn-In +6 more
openaire +2 more sources
PCR Mutagenesis-Based Method for Generation of Positive Controls for SSCP Analysis
BioTechniques, 1997 We have developed a primer-mediated PCR mutagenesis-based method for the generation of positive controls to test the sensitivity of single-strand conformation polymorphism (SSCP) or any other PCRbased mutation screening method. This technique is based on
A. Ruiz +4 more
doaj +1 more source
Analysis of the p53 gene by PCR-SSCP in ten cases of Wilms’ tumor [PDF]
Sao Paulo Medical Journal, 2000 CONTEXT: Mutations of the p53 tumor suppressor gene are the most frequent alterations observed in human neoplasias affecting adults. In pediatric oncology, however, they have seldom been identified. Wilms’ tumor is a renal neoplasia commonly occurring in children and is associated with mutations of the WT1 gene. The correlation between Wilms’ tumor and
Ricardo Defavery +6 more
openaire +6 more sources
BMC Medical Genetics, 2002 Background Single-nucleotide polymorphisms (SNPs) are considered to be useful polymorphic markers for genetic studies of polygenic traits. Single-stranded conformational polymorphism (SSCP) analysis has been widely applied to detect SNPs, including point
Taniyama Matsuo +4 more
doaj +1 more source
Acta Biológica Colombiana, 2015 Potato yellow vein virus (PYVV) with tripartite RNA (ss+) genome is classified as Crinivirus within the family Closteoviridae. PYVV is the causal agent of potato yellow vein disease (PYVD) with yield effect.
Karen Andrea CUBILLOS ABELLO +1 more
doaj +1 more source
[The application of PCR-SSCP in forensic mtDNA typing].
Fa yi xue za zhi, 2010 To study the application of PCR-SSCP in forensic mtDNA typing.Primers flanking the mtDNA HV-I and HV-II regions were designed. By PCR-SSCP techniques, 70 family trios and 140 unrelated Wuhan Han individuals were investigated and analyzed.In 70 family trios, the SSCP profiles in region HV-I and HV-II of children were not same to that of their fathers in
Hong-Wei, Ai +6 more
openaire +2 more sources