Results 71 to 80 of about 11,641 (223)
Abstract The future survival of coral reefs in the Anthropocene depends on the capacity of corals to adapt as oceans warm and extreme weather events become more frequent. Targeted interventions designed to assist evolutionary processes in corals require a comprehensive understanding of the distribution and structure of standing variation, however ...
Jia Zhang +8 more
wiley +1 more source
【目的】探讨用聚合酶链反应-单链构象多态性( PCR-SSCP)方法快速诊断结核分支杆菌链霉素( Sm)耐药的临床意义。【方法】用 PCR-SSCP 技术检测了 30 株Sm 敏感的结核分支杆菌临床分离株, 30 株 Sm 耐药的临床分离株,以结核分支杆菌 H37RV 标准株作对照。先用 PCR 方法扩增 rpsL 基因,随后用SSCP 方法鉴定其扩增产物有无突变。【结果】所有临床分离株均观察到 rpsL 基因 PCR 扩增产物。30 株 Sm 敏感的临床分离株与 H37RV 标准株呈现一样 SSCP ...
吴为群, 谢灿茂
doaj
High-Throughput Asymmetric-PCR SSCP Analysis Using Well-Controlled Temperature Conditions
Single-stranded conformational polymorphism (SSCP) analysis is useful for mutation detection and polymorphism identification. To realize high-throughput SSCP analysis using a high-intensity electric field, the gel temperature must be well-controlled.
Masaharu Kiyama, Takeshi Fujita
doaj +1 more source
Studying VSX1 gene mutations in patients with Keratoconus of Chaharmahal and Bakhtiari province, Iran [PDF]
Background & Aims: Keratoconus (KC) is an eye disorder in which the cornea is swollen, thinned and deformed. Despite extensive studies, the pathophysiological processes and genetic etiology of KC is unknown.
Azadegan-Dehkordi, Fatemeh. +11 more
core
Twenty‐three years of PCR‐based seafood authentication assay development: What have we learned?
Abstract Seafood is a prime target for fraudulent activities due to the complexity of its supply chain, high demand, and difficult discrimination among species once morphological characteristics are removed. Instances of seafood fraud are expected to increase due to growing demand.
Maleeka Singh +5 more
wiley +1 more source
Screening LRTOMT gene (DFNB63 locus) in patients with recessive nonsyndromic hearing loss in hormozgan province, Iran [PDF]
Background: Congenital hearing loss is the most common sensory disorder is modern societies. Two modes of syndromic and nonsyndromic genetic hearing loss can be seen.
Hashemzadeh-Chaleshtori, Morteza. +4 more
core
The relative influence of the microevolutionary influencing population genetic structure in highly mobile organisms is poorly understood. Here we analyze genetic variation in a coastal seabird from the northeast Pacific Ocean. We find that, unlike most high latitude, Northern Hemisphere seabirds, pigeon guillemots display significant population genetic
Bronwyn A. S. Harkness +3 more
wiley +1 more source
Single Strand Conformation Polymorphism analysis of PCR-amplified rDNA to differentiate medically important Aspergillus Species [PDF]
"nBackground: Aspergillus species are associated with allergic bronchopulmonary disease, mycotic keratitis, otomycosis, na­sal sinusitis and invasive infection.
K Diba +3 more
doaj +2 more sources
In the present study, we investigate three candidate genes for behavioural traits in the Belgian Malinois, aiming to identify genetic variation that influences behavioural traits, for example, trainability. Our finding suggests that the TAC1 gene variants could influence transcription factor binding and, subsequently, the trainability and excitability ...
Mohammad Fallahi +2 more
wiley +1 more source
GENETIC VARIATIONS IN THE MAPK 15 GENE AND THEIR RELATIONSHIP WITH MILK PRODUCTION CHARACTERISTICS IN MURRAH BUFFALO [PDF]
The current research explored the link between genetic diversity in the Mitogen-activated protein kinase 15 (MAPK 15) gene of buffalo and the traits related to milk composition and production, employing PCR-SSCP and Sanger sequencing techniques.
Sapna Nath +6 more
doaj +1 more source

