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2010
Die vielfaltigen Anwendungsmoglichkeiten der Polymerasekettenreaktion (polymerase chain reaction, PCR) machen sie zu einer der wichtigsten und am haufigsten eingesetzten Methoden in der molekularbiologischen Forschung und Diagnostik. Fur diese Technologie wurde der Erfinder der Methode, Kary Mullis, 1993 mit dem Nobelpreis ausgezeichnet.
Regina Konrad, Ulrich Busch
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Die vielfaltigen Anwendungsmoglichkeiten der Polymerasekettenreaktion (polymerase chain reaction, PCR) machen sie zu einer der wichtigsten und am haufigsten eingesetzten Methoden in der molekularbiologischen Forschung und Diagnostik. Fur diese Technologie wurde der Erfinder der Methode, Kary Mullis, 1993 mit dem Nobelpreis ausgezeichnet.
Regina Konrad, Ulrich Busch
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CODEHOP PCR and CODEHOP PCR Primer Design
2010While PCR primer design for the amplification of known sequences is usually quite straightforward, the design, and successful application of primers aimed at the detection of as yet unknown genes is often not. The search for genes that are presumed to be distantly related to a known gene sequence, such as homologous genes in different species, paralogs
Jeannette P, Staheli +3 more
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Direct PCR of Intact Bacteria (Colony PCR)
Current Protocols in Microbiology, 2008AbstractThis protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using polymerase chain reaction (PCR). This method is commonly referred to as colony PCR. Curr. Protoc. Microbiol. 9:A.3D.1‐A.3D.6. © 2008 by John Wiley & Sons, Inc.
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Long Range PCR (Long Template PCR, LA PCR)
2007Metodom LA PCR moguće je dobiti znatno dulje PCR produkte od onih umnoženih Taq DNA-polimerazom (Cheng i sur, 1994). Postiže se viši prinos PCR produkata i veća točnost prijepisa DNA slijeda (engl. fidelity). Pri tom se najčešće koristi smjesa Taq DNA-polimeraze i termostabilne DNA-polimeraze koja posjeduje 3'- 5' egzonukleaznu aktivnost (engl ...
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