Results 1 to 10 of about 6,499 (163)

Mutations in ampD cause hyperproduction of AmpC and CmcB β-lactamases and high resistance to β-lactam antibiotics in Chromobacterium violaceum [PDF]

Microbiology Spectrum
Bacterial resistance to β-lactam antibiotics mediated by β-lactamase enzymes is widespread worldwide. Chromobacterium violaceum, an environmental Gram-negative bacterial pathogen, is intrinsically resistant to some β-lactam antibiotics.
Luís Gustavo Laranjeiro, Carlos Eduardo M. Neme, Maristela Previato-Mello, Bianca B. Batista, Isabel Henriques, José F. da Silva Neto   +5 more
doaj   +2 more sources

Stabilisation and immobilisation of penicillin amidase

FEBS Letters, 1989
Penicillin amidase was coupled to a periodate‐oxidised dextran by reductive alkylation in the presence of sodium cyanoborohydride. A loss of activity (25%) was observed but the conjugate enzyme dextran was more thermostable than the native enzyme. Native and dextran‐conjugated penicillin amidase were immobilised on amino activated silica (Promaxon ...
R R Crichton, R R Crichton
exaly   +3 more sources

Effect of the kanamycin resistance marker on stability of 2μ-based expression plasmids [PDF]

Archives of Biological Sciences, 2007
In this paper we describe the effect of the kanamycin resistance gene (Kmr) on 2μm-based plasmid maintenance in Saccharomyces cerevisiae. The influence of this marker gene on the loss of the stable model-vectors proved to be constant, as well as ...
Stanković Nada, Vasiljević Branka, Ljubijankić Goran   +2 more
doaj   +1 more source

Probiotic Properties and Proteomic Analysis of Pediococcus pentosaceus 1101

Foods, 2022
Pediococcus pentosaceus 1101 was identified by using 16S rRNA and MALDI-Biotyper. The strain was exposed to conditions that resemble the gastrointestinal tract (GT) to evaluate its probiotic properties.
Monserrat Escobar-Sánchez, Ulises Carrasco-Navarro, Carmen Juárez-Castelán, Luis Lozano-Aguirre Beltrán, M. Lourdes Pérez-Chabela, Edith Ponce-Alquicira   +5 more
doaj   +1 more source

Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats

Frontiers in Veterinary Science, 2021
Caseous lymphadenitis (CLA) is a chronic disease that affects small ruminants and causes economic losses in the associated breeding system. The causative agent of CLA is Corynebacterium pseudotuberculosis, a Gram-positive bacterium that exhibits tropism ...
Maria Conceição Aquino de Sá, Wanderson Marques da Silva, Carla Catarine Santos Rodrigues, Cristiana Perdigão Rezende, Silvana Beutinger Marchioro, José Tadeu Raynal Rocha Filho, Thiago de Jesus Sousa, Helinando Pequeno de Oliveira, Mateus Matiuzzi da Costa, Henrique César Pereira Figueiredo, Ricardo Dias Portela, Thiago Luiz de Paula Castro, Vasco Azevedo, Nubia Seyffert, Roberto Meyer   +14 more
doaj   +1 more source

Exploitation of E. coli for the production of penicillin G amidase: a tool for the synthesis of semisynthetic β-lactam antibiotics

Journal of Genetic Engineering and Biotechnology, 2021
Background Penicillin G amidase/acylases from microbial sources is a unique enzyme that belongs to the N-terminal nucleophilic hydrolase structural superfamily.
Krishika Sambyal, Rahul Vikram Singh
doaj   +1 more source

Application of in vivo induced antigen technology (IVIAT) to Bacillus anthracis. [PDF]

PLoS ONE, 2008
In vivo induced antigen technology (IVIAT) is an immuno-screening technique that identifies bacterial antigens expressed during infection and not during standard in vitro culturing conditions.
Sean M Rollins, Amanda Peppercorn, John S Young, Melissa Drysdale, Andrea Baresch, Margaret V Bikowski, David A Ashford, Conrad P Quinn, Martin Handfield, Jeffrey D Hillman, C Rick Lyons, Theresa M Koehler, Stephen B Calderwood, Edward T Ryan   +13 more
doaj   +1 more source

Image-based dynamic phenotyping reveals genetic determinants of filamentation-mediated beta-lactam tolerance [PDF]

, 2020
Antibiotic tolerance characterized by slow killing of bacteria in response to a drug can lead to treatment failure and promote the emergence of resistance.
Camacho, Rafael, Fauvart, Maarten, Franke, Sabine, Hofkens, Johan, Marchal, Kathleen, Michielsen, Jan, Weytjens, Bram, Wilmaerts, Dorien, Zahir, Taiyeb   +8 more
core   +2 more sources

The absence of a mature cell wall sacculus in stable Listeria monocytogenes L-form cells is independent of peptidoglycan synthesis [PDF]

, 2016
L-forms are cell wall-deficient variants of otherwise walled bacteria that maintain the ability to survive and proliferate in absence of the surrounding peptidoglycan sacculus.
Ayala, Juan A, Borisova, Marina, Briers, Yves, Loessner, Martin J, Mayer, Christoph, Schneider, Alexander, Schuppler, Markus, Studer, Patrick   +7 more
core   +9 more sources

Structural and Functional Insights into Peptidoglycan Access for the Lytic Amidase LytA of Streptococcus pneumoniae

mBio, 2014
The cytosolic N-acetylmuramoyl-l-alanine amidase LytA protein of Streptococcus pneumoniae, which is released by bacterial lysis, associates with the cell wall via its choline-binding motif.
Peter Mellroth, Tatyana Sandalova, Alexey Kikhney, Francisco Vilaplana, Dusan Hesek, Mijoon Lee, Shahriar Mobashery, Staffan Normark, Dmitri Svergun, Birgitta Henriques-Normark, Adnane Achour   +10 more
doaj   +1 more source