Results 21 to 30 of about 6,518 (178)

One-step-purification of penicillin G amidase from cell lysate using ion-exchange membrane adsorbers [PDF]

, 2013
This study describes the purification of penicillin G amidase (PGA) by ion exchange membrane adsorbers in a one-step-process. Preliminary experiments with high-throughput screening devices in microliter scale (8-strip modules) were performed to find ...
Beutel, Sascha, Mönster, Andreas, Scheper, Thomas, Villain, Louis   +3 more
core   +2 more sources

A novel aryl acylamidase from Nocardia farcinica hydrolyses polyamide [PDF]

, 2009
An alkali stable polyamidase was isolated from a new strain of Nocardia farcinica. The enzyme consists of four subunits with a total molecular weight of 190 kDa.
Almansa, Asatekin, Cai, Ciskanik, Cramp, De Geyter, Deguchi, Fischer-Colbrie, Fournand, Friedrich, Fujisawa, Gudelj, Guebitz, Hammond, Hayashi, Heumann, Hirrlinger, Hwang, Ishikawa, Jaganathan, Kakudo, Kim, Klun, Krieg, Labahn, Li, Lowry, McCord, Meyer, Negoro, Negoro, Nomura, Qiao, Saitou, Silva, Skouloubris, Thompson, Toogood, Tusek, Valina, Vertommen, Wu, Yoshioka   +42 more
core   +1 more source

Proinflammatory bacterial peptidoglycan as a cofactor for the development of central nervous system autoimmune disease [PDF]

, 2005
Upon stimulation by microbial products through TLR, dendritic cells (DC) acquire the capacity to prime naive T cells and to initiate a proinflammatory immune response.
Boven, L.A. (Leonie), Heer, H.J. de, Laman, J.D. (Jon), Lambrecht, B.N.M. (Bart), Melief, M.J. (Marie-José), Meurs, M. (Marjan) van, Nieuwenhuis, E.E.S. (Edward), Riel, D.A.J. (Debby) van, Strijp, J. van, Visser, L. (Lizette), Zahringer, U.   +10 more
core   +2 more sources

Bacterial Cell Enlargement Requires Control of Cell Wall Stiffness Mediated by Peptidoglycan Hydrolases. [PDF]

, 2015
Most bacterial cells are enclosed in a single macromolecule of the cell wall polymer, peptidoglycan, which is required for shape determination and maintenance of viability, while peptidoglycan biosynthesis is an important antibiotic target.
Bailey, R.G., Foster, S.J., Hayhurst, E.J., Hobbs, J.K., Horsburgh, M., Mesnage, S., Mohamad, S.A., Salamaga, B., Turner, R.D., Wheeler, R.   +9 more
core   +3 more sources

CodY, a pleiotropic regulator, influences multicellular behaviour and efficient production of virulence factors in Bacillus cereus [PDF]

, 2012
In response to nutrient limitation in the environment, the global transcriptional regulator CodY modulates various pathways in low G+C Gram-positive bacteria.
Basset, Coraline, Granum, Per Einar, Kovacs, Akos T., Kuipers, Oscar P., Lindback, Toril, Lindbäck, Toril, Mols, Maarten   +6 more
core   +6 more sources

Staphylococcus aureus Survives with a Minimal Peptidoglycan Synthesis Machine but Sacrifices Virulence and Antibiotic Resistance [PDF]

, 2015
Many important cellular processes are performed by molecular machines, composed of multiple proteins that physically interact to execute biological functions. An example is the bacterial peptidoglycan (PG) synthesis machine, responsible for the synthesis
A Antignac, A Bera, A Typas, AM Jorge, Andreas Peschel, AR Pereira, AW Wyke, B Langmead, BJ Hartman, CI Chang, DC McPherson, DJ Scheffers, DY Wu, E Kuru, Egbert Hoiczyk, G Memmi, G Sakoulas, H Komatsuzawa, H Komatsuzawa, H Labischinski, HW Boucher, I Kounatidis, IG Boneca, IG Boneca, IG Boneca, J Gough, José B. Pereira-Leal, JV Holtje, JV Holtje, LB Rice, M El Ghachi, M Kuroda, M Kuroda, M Vicente, M Wu, Magda L. Atilano, Mariana G. Pinho, MG Pinho, MG Pinho, MG Pinho, MG Pinho, MG Pinho, ML Atilano, ML Atilano, NAC Curtis, Nathalie T. Reichmann, NW Goehring, P Giesbrecht, P Matthews, P Reed, Patricia Reed, Pedro M. Pereira, Petros Ligoxygakis, PM Coutinho, R Tam, RA Forsyth, Renato Alves, S Borukhov, S Guindon, SFF Pereira, SFF Pereira, SR Eddy, SR Filipe, Sérgio R. Filipe, T Michel, Terry Roemer, TF Mackay, VR Steele, W Strobel, X Fan, Xinwei Sher, Z Kelman   +71 more
core   +4 more sources

Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell [PDF]

, 2018
Biopolymer composite cell walls maintain cell shape and resist forces in plants, fungi and bacteria. Peptidoglycan, a crucial antibiotic target and immunomodulator, performs this role in bacteria.
A Typas, AJ Meeske, AL Koch, AW Bisson-Filho, C Leung, C Paradis-Bleau, CN Takacs, D Bradley, E Rojas, EJ Hayhurst, F Wong, GJ Bean, H Harz, H Labischinski, J Heijenoort van, JC Gumbart, KC Huang, KD Young, L Furchtgott, L Gan, LT Nguyen, M Wachi, MM Farley, R Wheeler, R Wheeler, RD Turner, RD Turner, RD Turner, RD Turner, RS Dover, RW Verwer, S Hussain, S Teeffelen van, SR Clarke, T Kruse, T Pilizota, TS Ursell, V Braun, X Yang, X Yao, YF Dufrêne   +40 more
core   +2 more sources

A Mouse Amidase Specific for N-terminal Asparagine: the gene, the enzyme, and their function in the N-end rule pathway [PDF]

, 1996
The N-end rule relates the in vivo half-life of a protein to the identity of its N-terminal residue. In both fungi and mammals, the tertiary destabilizing N-terminal residues asparagine and glutamine function through their conversion, by enzymatic ...
Arfin, Stuart M., Bradshaw, Ralph A., Copeland, Neal G., Grigoryev, Sergei, Jenkins, Nancy A., Kwon, Yong Tae, Stewart, Albert E., Varshavsky, Alexander   +7 more
core  

Comprehensive and Comparative Transcriptional Profiling of the Cell Wall Stress Response in Bacillus subtilis

Molecular Microbiology, EarlyView.
Transcriptional profiling of the primary cell envelope stress response (CESR) of Bacillus subtilis exposed to a range of cell wall‐targeting antimicrobials, combining RNAseq and high‐resolution tiling arrays. We revealed the complexity of the CESR, the contribution of extracytoplasmic function sigma factors (ECFs) and two‐component signal transduction ...
Qian Zhang, Charlène Cornilleau, Lena Friebel, Sarah Spitzner, Raphael R. Müller, Doreen Meier, Pierre Flores, Cyprien Guérin, Pierre Nicolas, Diana Wolf, Vincent Fromion, Rut Carballido‐López, Thorsten Mascher   +12 more
wiley   +1 more source

A Cathepsin B‐Triggered CO‐Releasing Molecule with a Non‐Toxic Metal Core for Targeted Tumor Delivery

Angewandte Chemie, Volume 138, Issue 1, 2 January 2026.
An iron‐based, enzyme‐triggered CO‐releasing molecule (ET‐CORM) is site‐specifically conjugated to the antibody trastuzumab. This novel ET‐CORM‐antibody conjugate releases carbon monoxide upon cleavage by the tumor‐associated protease cathepsin B. The construct enables selective intracellular CO delivery to HER2‐overexpressing and CatB‐expressing cells,
Inga Černauskienė, Eduardo Izquierdo‐García, Sarah Keller, Harley Betts, Kevin Cariou, Vicente Marchán, Gilles Gasser, Gonçalo J. L. Bernardes   +7 more
wiley   +2 more sources