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Plant Molecular Biology, 2014
Inflorescence architecture, pedicel length and stomata patterning in Arabidopsis thaliana are specified by inter-tissue communication mediated by ERECTA and its signaling ligands in the EPIDERMAL PATTERNING FACTOR-LIKE (EPFL) family of secreted cysteine-rich peptides. Here, we identified and characterized BnEPFL6 from Brassica napus.
Yi, Huang +6 more
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Inflorescence architecture, pedicel length and stomata patterning in Arabidopsis thaliana are specified by inter-tissue communication mediated by ERECTA and its signaling ligands in the EPIDERMAL PATTERNING FACTOR-LIKE (EPFL) family of secreted cysteine-rich peptides. Here, we identified and characterized BnEPFL6 from Brassica napus.
Yi, Huang +6 more
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Journal of biochemistry, 1991
Peptide elongation factor 3 (EF-3), which is widely present in yeasts and fungi (Eumycota), does not occur in another lower eukaryote, the unicellular protozoan Tetrahymena pyriformis, as was shown by the following findings: (a) there is no activity to satisfy the EF-3 requirement of yeast ribosomes in the post-ribosomal supernatant fraction from ...
M, Miyazaki, H, Kagiyama
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Peptide elongation factor 3 (EF-3), which is widely present in yeasts and fungi (Eumycota), does not occur in another lower eukaryote, the unicellular protozoan Tetrahymena pyriformis, as was shown by the following findings: (a) there is no activity to satisfy the EF-3 requirement of yeast ribosomes in the post-ribosomal supernatant fraction from ...
M, Miyazaki, H, Kagiyama
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Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1997
The cDNA for a homologue of elongation factor Ts which probably functions in mitochondria has been sequenced from a nematode Caenorhabditis elegans. The deduced amino acid sequence (316 amino acids long) has a possible transit peptide sequence at the amino terminus and several common specific features for mammalian mitochondrial EF-Ts.
Y, Watanabe +3 more
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The cDNA for a homologue of elongation factor Ts which probably functions in mitochondria has been sequenced from a nematode Caenorhabditis elegans. The deduced amino acid sequence (316 amino acids long) has a possible transit peptide sequence at the amino terminus and several common specific features for mammalian mitochondrial EF-Ts.
Y, Watanabe +3 more
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European Journal of Biochemistry, 1988
A procedure is described for the purification of the archaebacterial peptide‐elongation factor 2 (aEF‐2) from an extremely halophilic archaebacterium Halobacterium halobium. The enrichment was about 530‐fold, the obtained preparation practically homogeneous as judged by SDS‐PAGE. The poly(U)‐dependent poly(Phe) synthesis was completely dependent on aEF‐
H, Saruyama, S, Sasaki
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A procedure is described for the purification of the archaebacterial peptide‐elongation factor 2 (aEF‐2) from an extremely halophilic archaebacterium Halobacterium halobium. The enrichment was about 530‐fold, the obtained preparation practically homogeneous as judged by SDS‐PAGE. The poly(U)‐dependent poly(Phe) synthesis was completely dependent on aEF‐
H, Saruyama, S, Sasaki
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1971
Publisher Summary This chapter describes the assay methods for amino acid polymerization, GTP hydrolysis, formation of a GTP-protein complex, and the GTP-dependent binding of phenylalanyl-tRNA to ribosomes; the isolation of the supernatant fluid containing the elongation factors FI and FII from yeast cell; the separation of the elongation factors ...
Dietmar Richter, Friedrich Klink
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Publisher Summary This chapter describes the assay methods for amino acid polymerization, GTP hydrolysis, formation of a GTP-protein complex, and the GTP-dependent binding of phenylalanyl-tRNA to ribosomes; the isolation of the supernatant fluid containing the elongation factors FI and FII from yeast cell; the separation of the elongation factors ...
Dietmar Richter, Friedrich Klink
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Role of yeast peptide elongation factor 3 (EF-3) at the AA-tRNA binding step.
Journal of biochemistry, 1989The stimulatory effect of peptide elongation factor 3 (EF-3), which is uniquely required for the yeast elongation cycle, on the step of binding of aminoacyl-tRNA (AA-tRNA) to ribosomes has been investigated in detail. Yeast EF-1 alpha apparently functions in a stoichiometric manner in the binding reaction of AA-tRNA to the ribosomes. The addition of EF-
M, Uritani, M, Miyazaki
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Separation of mitochondrial and cytoplasmic peptide chain elongation factors from yeast
Biochemistry, 1970D, Richter, F, Lipmann
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Biokhimiia (Moscow, Russia), 1983
It is shown that preparations of EF-T are practically free from GTPase activity, if they were formed from EF-Tu and EF-Ts carefully purified from GTPase beforehand. Some Ef-T-dependent GTPase can arise after addition of tRNA or aminoacyl-tRNA preparations to a EF-T+ribosomes mixture.
S K, Smailov +2 more
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It is shown that preparations of EF-T are practically free from GTPase activity, if they were formed from EF-Tu and EF-Ts carefully purified from GTPase beforehand. Some Ef-T-dependent GTPase can arise after addition of tRNA or aminoacyl-tRNA preparations to a EF-T+ribosomes mixture.
S K, Smailov +2 more
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Bioorganicheskaia khimiia, 1984
Peptides obtained as a result of cyanogen bromide cleavage of the G-factor have been studied. All 12 peptides embracing the whole structure of fragment T4 have been isolated. For their amino acid sequence determination, cyanogen bromide peptides have been further cleaved with trypsin, chymotrypsin, thermolysin, staphylococcal glutamic protease and BNPS-
Iu B, Alakhov +4 more
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Peptides obtained as a result of cyanogen bromide cleavage of the G-factor have been studied. All 12 peptides embracing the whole structure of fragment T4 have been isolated. For their amino acid sequence determination, cyanogen bromide peptides have been further cleaved with trypsin, chymotrypsin, thermolysin, staphylococcal glutamic protease and BNPS-
Iu B, Alakhov +4 more
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Biochemistry and molecular biology international, 1994
As it has been found, the incubation of [gamma-32P]ATP with elongation factor--1 alpha purified from rabbit reticulocytes resulted in the phosphorylation of several substrate proteins /Tuhácková, Z. (1992) In: Rec. Adv. Cell. Mol. Biol. 4, 79-86, Peeters Press, Leuven/ (1).
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As it has been found, the incubation of [gamma-32P]ATP with elongation factor--1 alpha purified from rabbit reticulocytes resulted in the phosphorylation of several substrate proteins /Tuhácková, Z. (1992) In: Rec. Adv. Cell. Mol. Biol. 4, 79-86, Peeters Press, Leuven/ (1).
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