Results 51 to 60 of about 119,991 (264)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Anticipated discrimination in daily life: Predictors, stress appraisals, and responses.

PLoS ONE
A large body of literature details the deleterious effects of everyday discrimination on health, focusing on stress processes after discrimination occurs.
Lydia Q Ong, Megan W Wolk, Anthony L Burrow, Monika Lohani, Patrick L Hill, Nancy L Sin   +5 more
doaj   +1 more source

Perceived discrimination among tuberculosis patients in an urban area of Kolkata City, India

Journal of Global Infectious Diseases, 2020
Context and Aims: Tuberculosis (TB) in India is a leading public health problem plagued by social determinants such as stigma and discrimination, which may affect treatment seeking, adherence, and possibly treatment outcome.
Sitikantha Banerjee, Kajari Bandyopadhyay, Pranita Taraphdar, Aparajita Dasgupta   +3 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

Impact of perceived discrimination and coping strategies on well-being and mental health in newly-arrived migrants in Spain.

PLoS ONE, 2023
ObjectivesTo explore how perceived discrimination impacts the emotional well-being and mental health of newly-arrived migrants in Spain; and to identify the coping strategies and behavioral changes used to deal with perceived discrimination.Design102 ...
Aina Gabarrell-Pascuet, Amanda Lloret-Pineda, Marta Franch-Roca, Blanca Mellor-Marsa, Maria Del Carmen Alos-Belenguer, Yuelu He, Rachid El Hafi-Elmokhtari, Felipe Villalobos, Ivet Bayes-Marin, Lola Aparicio Pareja, Oscar Álvarez Bobo, Mercedes Espinal Cabezas, Yolanda Osorio, Josep Maria Haro, Paula Cristóbal-Narvaez   +14 more
doaj   +1 more source

Perceived Neighborhood Crime Safety Moderates the Association Between Racial Discrimination Stress and Chronic Health Conditions Among Hispanic/Latino Adults

Frontiers in Public Health, 2021
Background: Little is known about the link between perceived neighborhood walkability and prevalence of chronic disease. Even less is known regarding this association among Hispanic/Latino adults, despite exhibiting high rates of chronic diseases. Stress
Elizabeth L. Budd, Elizabeth L. Budd, Nicole R. Giuliani, Nicole R. Giuliani, Nichole R. Kelly, Nichole R. Kelly   +5 more
doaj   +1 more source

Perceived religious discrimination in healthcare

, 2023
Swedish healthcare providers must comply with the Patient Act's principles of equal and accessible care and account for patients’ religious backgrounds by offering culturally sensitive care. This chapter explores what characterizes patients’ and their relatives’ expectations in healthcare encounters perceived as religiously discriminatory in the ...
Eriksson, Lise, Dudas, Victor, Carlbom, Aje, Essén, Birgitta   +3 more
openaire   +3 more sources