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Electroporation Transformation Protocol for Phaeodactylum tricornutum
2019Genetic transformation system is very important for both basic biological research and commercial exploitation of diatoms. Here we describe a high-efficiency nuclear transformation method for the model diatom Phaeodactylum tricornutum using an electroporation system, and the maximum transformation frequency obtained is about 3 × 10-5 cells.
Hanhua, Hu, Yufang, Pan
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Chlorophyllase and Lamellar Structure in Phaeodactylum tricornutum
Zeitschrift für Pflanzenphysiologie, 1975Summary 1. Chlorophyllase in the marine diatom Phaeodactylum tricornutum is inactive, or nearly so, in vivo, but becomes active upon desintegration of the cell structure by osmotic shock or French Press treatment. Cell desintegration is apparent from disappearance of long-wave chlorophyll forms (685 and 710 nm), present in the intact algae (Goedheer,
Willemke Terpstra, J.C. Goedheer
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203Hg as tracer in pigments of phaeodactylum tricornutum
The International Journal of Applied Radiation and Isotopes, 1973Abstract Cells of Phaeodactylum tricornutum were exposed to 0·12 ppm Hg (containing 203Hg as a tracer in a 3·5% NaCl solution and in dilute culture medium of the same salinity. Radioactivity measurements indicated that mercury uptake by the cells was comparable in both solutions, and that less than 2% of the sorbed mercury was in the pigments, as ...
P J, Hannan +3 more
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A Review of Fucoxanthin Biomanufacturing from Phaeodactylum tricornutum
Bioprocess and Biosystems EngineeringMicroalgae, compared to macroalgae, exhibit advantages such as rapid growth rates, feasible large-scale cultivation, and high fucoxanthin content. Among these microalgae, Phaeodactylum tricornutum emerges as an optimal source for fucoxanthin production.
Yunlong, Pang +5 more
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Environmental evaluation of eicosapentaenoic acid production by Phaeodactylum tricornutum
Science of The Total Environment, 2014Polyunsaturated fatty acids (PUFAs) play an important role in human health. Due to the increased market demand, the production of PUFAs from potential alternative sources such as microalgae is receiving increased interest. The aim of this study was to perform a life cycle assessment (LCA) of the biotechnological production of eicosapentaenoic acid (EPA)
Perez-Lopez, Paula +6 more
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Effect of chromate ions on marine microalgae Phaeodactylum tricornutum
Microbiology, 2017Effect of chromate ions on the culture of a marine diatom Phaeodactylum tricornutum was studied using an M-PEA-2 fluorimeter, which carries out simultaneous measurement of fluorescence induction and redox transformations of the P700 pigment within a millisecond range. Chromate ions were shown to inhibit electron transport in PS II and decrease the rate
D T, Gabbasova +4 more
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Molecular toolbox for studying diatom biology in Phaeodactylum tricornutum
Gene, 2007Research into diatom biology has now entered the post-genomics era, following the recent completion of the Thalassiosira pseudonana and Phaeodactylum tricornutum whole genome sequences and the establishment of Expressed Sequence Tag (EST) databases. The thorough exploitation of these resources will require the development of molecular tools to analyze ...
Magali, Siaut +8 more
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Heterotrophic modification of Phaeodactylum tricornutum Bohlin
Algal Research, 2023Wenlei Zhang +6 more
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2014
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???????????????????? ???????????????????????? ?? ???????????????????????????? ???????????? ???????????????????????? ???????????????????? ???????????????????????????? Phaeodactylum tricornutum, ???????????????????????????? ?????? ???????????? ?????????????????????? ?? ????????????????????????. ??????????????????????, ?????? ?????????????????????????? ???
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2015
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?????????????????? ?????????????????????????????? ???????? ???? ???????????? ?????????????? ?? ?????????????????????? ???????????????????????????? Phaeodactylum tricornutum. ??????????????????????, ?????? ???????????????????? ??????????????????????, ???????????????????? ???????????????????? ?? ?? ???????????????????????? ????????????????????????????, ??
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