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Electroporation Transformation Protocol for Phaeodactylum tricornutum

2019
Genetic transformation system is very important for both basic biological research and commercial exploitation of diatoms. Here we describe a high-efficiency nuclear transformation method for the model diatom Phaeodactylum tricornutum using an electroporation system, and the maximum transformation frequency obtained is about 3 × 10-5 cells.
Hanhua, Hu, Yufang, Pan
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Chlorophyllase and Lamellar Structure in Phaeodactylum tricornutum

Zeitschrift für Pflanzenphysiologie, 1975
Summary 1. Chlorophyllase in the marine diatom Phaeodactylum tricornutum is inactive, or nearly so, in vivo, but becomes active upon desintegration of the cell structure by osmotic shock or French Press treatment. Cell desintegration is apparent from disappearance of long-wave chlorophyll forms (685 and 710 nm), present in the intact algae (Goedheer,
Willemke Terpstra, J.C. Goedheer
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203Hg as tracer in pigments of phaeodactylum tricornutum

The International Journal of Applied Radiation and Isotopes, 1973
Abstract Cells of Phaeodactylum tricornutum were exposed to 0·12 ppm Hg (containing 203Hg as a tracer in a 3·5% NaCl solution and in dilute culture medium of the same salinity. Radioactivity measurements indicated that mercury uptake by the cells was comparable in both solutions, and that less than 2% of the sorbed mercury was in the pigments, as ...
P J, Hannan   +3 more
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A Review of Fucoxanthin Biomanufacturing from Phaeodactylum tricornutum

Bioprocess and Biosystems Engineering
Microalgae, compared to macroalgae, exhibit advantages such as rapid growth rates, feasible large-scale cultivation, and high fucoxanthin content. Among these microalgae, Phaeodactylum tricornutum emerges as an optimal source for fucoxanthin production.
Yunlong, Pang   +5 more
openaire   +2 more sources

Environmental evaluation of eicosapentaenoic acid production by Phaeodactylum tricornutum

Science of The Total Environment, 2014
Polyunsaturated fatty acids (PUFAs) play an important role in human health. Due to the increased market demand, the production of PUFAs from potential alternative sources such as microalgae is receiving increased interest. The aim of this study was to perform a life cycle assessment (LCA) of the biotechnological production of eicosapentaenoic acid (EPA)
Perez-Lopez, Paula   +6 more
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Effect of chromate ions on marine microalgae Phaeodactylum tricornutum

Microbiology, 2017
Effect of chromate ions on the culture of a marine diatom Phaeodactylum tricornutum was studied using an M-PEA-2 fluorimeter, which carries out simultaneous measurement of fluorescence induction and redox transformations of the P700 pigment within a millisecond range. Chromate ions were shown to inhibit electron transport in PS II and decrease the rate
D T, Gabbasova   +4 more
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Molecular toolbox for studying diatom biology in Phaeodactylum tricornutum

Gene, 2007
Research into diatom biology has now entered the post-genomics era, following the recent completion of the Thalassiosira pseudonana and Phaeodactylum tricornutum whole genome sequences and the establishment of Expressed Sequence Tag (EST) databases. The thorough exploitation of these resources will require the development of molecular tools to analyze ...
Magali, Siaut   +8 more
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Heterotrophic modification of Phaeodactylum tricornutum Bohlin

Algal Research, 2023
Wenlei Zhang   +6 more
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?????????????? ?????????????? ?????????????????????????????? Phaeodactylum tricornutum Bohl. (Bacillariophyta) ???? ???????????????????? ????????????????????????

2014
???????????????????? ???????????????????????? ?? ???????????????????????????? ???????????? ???????????????????????? ???????????????????? ???????????????????????????? Phaeodactylum tricornutum, ???????????????????????????? ?????? ???????????? ?????????????????????? ?? ????????????????????????. ??????????????????????, ?????? ?????????????????????????? ???
openaire   +1 more source

???????????????????? ???????????????????????????? Phaeodactylum tricornutum Bohlin (Bacillariophyta) ?????? ???????????? ???????????????? ?????? ???????????? ?????????????? ?????????????????? ???????? (????????????)

2015
?????????????????? ?????????????????????????????? ???????? ???? ???????????? ?????????????? ?? ?????????????????????? ???????????????????????????? Phaeodactylum tricornutum. ??????????????????????, ?????? ???????????????????? ??????????????????????, ???????????????????? ???????????????????? ?? ?? ???????????????????????? ????????????????????????????, ??
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