Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
An Analysis of the Influence of DSC Parameters on the Measurement of the Thermal Properties of Phase-Change Material. [PDF]
Gao M, Zhao S, Yang H, Wu X, Xiao Y.
europepmc +1 more source
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
Thermal Performance of Wood Frame Construction with Phase Change Material in the Brazilian Subtropical Climate. [PDF]
Vieira J +5 more
europepmc +1 more source
Acute caffeine treatment protects the developing retina from ischemia‐induced cell death
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento +6 more
wiley +1 more source
Transition Boundary from Laminar to Turbulent Flow of Microencapsulated Phase Change Material Slurry-Experimental Results. [PDF]
Dutkowski K, Kruzel M, Kochanowska M.
europepmc +1 more source
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero +5 more
wiley +1 more source
Visible-Light-Controlled Thermal Energy Storage and Release: A <i>Tetra</i>-<i>Ortho</i>-Fluorinated Azobenzene-Doped Composite Phase Change Material. [PDF]
Zhang Y, Qi J, Xia J, Zhai F, Dong L.
europepmc +1 more source
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin +3 more
wiley +1 more source
Leakage-Proof and High-Conductivity Composite Phase Change Material Using Low-Melting-Point-Alloy-Encapsulated Copper Foam/Paraffin for Superior Thermal Homogeneity in Lithium-Ion Battery Modules. [PDF]
He S, Zhao J, Ouyang D, Chen M.
europepmc +1 more source

