Results 141 to 150 of about 5,552,824 (295)

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source

Figure 2: Path-Gradient-Planning - Situational Gradient Norms

Path-Gradient Norms for Grid-world environments show in Figure 1.E-OEach frame represents the heat-map of gradient norms for each state over training steps. Gradients were computed using Path-Gradient Planning with situational focus.
Francesco Trapani (16642515)
core   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Heparan sulfate-dependent phase separation of CCL5 and its chemotactic activity

eLife
Secreted chemokines form concentration gradients in target tissues to control migratory directions and patterns of immune cells in response to inflammatory stimulation; however, how the gradients are formed is much debated.
Xiaolin Yu, Guangfei Duan, Pengfei Pei, Long Chen, Renji Gu, Wenrui Hu, Hongli Zhang, Yan-Dong Wang, Lili Gong, Lihong Liu, Ting-Ting Chu, Jin-Ping Li, Shi-Zhong Luo   +12 more
doaj   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

The Anti-Phase Gradient Operator in Anti-Mathematics: A Phase-Rotated Extension of Gradient Analysis

This article introduces the anti-phase gradient operator as a nonlinear phase-structured extension of gradient analysis within the broader Anti-Mathematics and Anti-Calculus framework. Instead of defining an anti-gradient merely as the negative gradient, the proposed operator maps the local gradient into a phase-modulated vector field.
openaire   +2 more sources

Controllable design and modeling of gradient porous structures by phase field theory

eXPRESS Polymer Letters
In this study, a novel methodology for the fabrication of gradient porous structures is introduced, predicated upon the phase evolution characteristics of immiscible polymer blends. Initially, a comprehensive flow-phase field dynamics model is developed.
Hangming Shen, Xingzhe Zhao, Lihong Yang, Jiantao Yang   +3 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Time-Gradient Phase-Transition Gravity (T-PTG)

This project introduces Time-Gradient Phase-Transition Gravity (T-PTG), a five-dimensional scalar-gravity theory designed to unify Modified Newtonian Dynamics (MOND) phenomenology, cosmological constant suppression, and quantum gravitational consistency ...
Hironori Suzawa
core   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source