Results 111 to 120 of about 16,689,295 (329)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Strong effect of precursor preparation on the morphology of semicrystalline phase inversion poly(vinylidene fluoride) membranes

open access: yes, 2009
This work documents the role of precursor preparation as an overlooked important factor in the control of membrane structures obtained by the phase inversion (PI) of solutions of crystallizable polymers.
Lin, Dar-jong; Beltsios, Konstantinos; Young, Tai-horng; Jeng, Yi-su; Cheng, Liao-ping   +1 more
core   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

A Novel Inversion Method of Manufacturing Flaws in the Packaging of Conformal Load-Bearing Antenna Structure

open access: yesInternational Journal of Antennas and Propagation, 2015
Composite material is widely used in the conformal load-bearing antenna structure (CLAS), and the manufacturing flaws in the packaging process of the CLAS will lead to the degradation of its wave-transparent property.
P. Li, W. Y. Xu, L. W. Song, Y. Y. Qiu
doaj   +1 more source

TRPML1 agonist ML‐SA5 attenuates pulmonary fibroblast activation by suppressing mTOR and restoring autophagic flux

open access: yesFEBS Open Bio, EarlyView.
TGF‐β1 stimulation downregulates lysosomal channel TRPML1 in pulmonary fibroblasts. The TRPML1 agonist ML‐SA5 suppressed fibroblast‐to‐myofibroblast activation and collagen production. Mechanistically, ML‐SA5 inhibited mTOR phosphorylation, restored autophagic flux, and its effects were enhanced by rapamycin (mTOR inhibitor) and reversed by MHY1485 ...
Jiatong Yao   +10 more
wiley   +1 more source

Multi-phase System Supplied by SVM VSI: A New Fast Algorithm to Compute Duty Cycles [PDF]

open access: yes, 2004
La version éditeur de cette article est disponible à l'adresse suivante : http://www.epe-association.org/epe/index.phpMany authors proposed SVM VSI applied to multi-phase ...
HAUTIER, Jean-Paul   +2 more
core   +1 more source

Solution of one phase Inverse Stefan Problem by IEF method

open access: yesJournal of Physics: Conference Series, 2014
A solution for Inverse Stefan Problem is represented in explicit analytic form. The developed method is based on use of Integral Error Functions and its properties which enables it to solve heat and mass transfer problems with domains that include moving boundaries.
M M Sarsengeldin, S Guvercin, S Kassabek
openaire   +1 more source

SPG4 and Dementia: Expanding the Clinical Spectrum

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Hereditary spastic paraplegia (HSP) is a group of disorders characterized by progressive spasticity and lower limb weakness, with mutations in SPG4/SPAST being the most common cause. Detailed studies and clinical and molecular comparisons across different populations are missing.
Emanuele Panza   +19 more
wiley   +1 more source

Pine Forest Height Inversion Using Single-Pass X-Band Pol-InSAR Data [PDF]

open access: yes, 2007
International audienceA sparse pine forest is investigated at X-band on a single-pass polarimetric synthetic aperture radar interferometry (PolInSAR) data set using HH and HV channels.
Dubois-Fernandez, Pascale C.   +4 more
core   +1 more source

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