Results 21 to 30 of about 114,055 (252)

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Membrane sampling separates naphthenic acids from biogenic dissolved organic matter for direct analysis by mass spectrometry

, 2022
This is a peer-reviewed accepted manuscript version of an article that was published as: Duncan, K.D., Hawkes, J.A., Berg, M., Clarijs, B., Gill, C.G., Bergquist, J., Lanekoff, I., & Krogh, E.T. (2022).
Duncan, Kyle D.,, Gill, Chris G.,, Krogh, Erik T., Clarijs, Bas, Bergquist, Jonas, Berg, Mykelti,, Hawkes, Jeffrey A.,, Duncan, Kyle D., Clarijs, Bas,, Gill, Chris G., Hawkes, Jeffrey A., Berg, Mykelti, Lanekoff, Ingela, Lanekoff, Ingela,, Bergquist, Jonas,, Krogh, Erik T.,   +15 more
core   +1 more source

Hybrid Classical–Post-Quantum Migration Framework for Brownfield SCADA Environments

IEEE Access
Brownfield supervisory control and data acquisition environments face a migration problem that is materially different from enterprise information technology.
Ankit Gupta, Shilpi Mittal
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

IMPACT OF NON-SOLVENT INDUCED PHASE SEPARATION ON CELLULOSE AEROGEL MORPHOLOGY

, 2023
International audienceAerogels from biomass, especially from polysaccharides, are of great interest due their good mechanical properties, general biocompatibility, biodegradability, and chemical tailorability for specific applications (adsorption of drug
Budtova, Tatiana, Gurikov, Pavel, Gelas, Loris   +2 more
core  

Recommendations for Automating Hydrogen/Deuterium Exchange Mass Spectrometry Measurements using Data-Independent Acquisition Methods

bioRxiv
Hydrogen/deuterium exchange mass spectrometry (HX-MS) is a method used to study solution-phase protein structure and dynamics. Despite its many applications, HX-MS is limited in throughput because manual data analysis is still the norm.
Frantisek Filandr, Morgan Hepburn, V. Sarpe, D. Crowder, Maryam Hassannia, Stephen Coales, Yuqi Shi, Rosa Viner, M. Rossotti, Joey G. Sheff, J. Tanha, D. Schriemer   +11 more
semanticscholar   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

First-order multi-k phase transitions and magnetoelectric effects in multiferroic Co3TeO6

, 2012
A theoretical description of the sequence of magnetic phases in Co3TeO6 is presented. The strongly first-order character of the transition to the commensurate multiferroic ground state, induced by coupled order parameters corresponding to different wave ...
Khalyavin D, Ivanov, Sergey A.,, Ivanov S, Lottermoser T, Fiebig, Manfred,, Khalyavin, Dmitry D.,, Carolus V, Lottermoser, Thomas,, Carolus, Vera,, Toledano, Pierre,, Tolédano P, Fiebig M, Hudl, Matthias,, Hudl M   +13 more
core   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells

Molecular Oncology, EarlyView.
This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos, Gilar Gorji‐bahri, Lejla Gradjan, Julia Zajac, Kacper Gil, Yvonne Thokozile Nduku, Anna M. Blom   +6 more
wiley   +1 more source