Results 241 to 250 of about 24,839 (278)
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Mannitol acetyl phosphate phosphotransferase of Aspergillus
Biochemical and Biophysical Research Communications, 1967Abstract An Aspergillus strain that accumulates free mannitol in a glucose medium has been previously reported Lee 1967. This organism also grows in a medium with mannitol as the sole source of carbon. In studying the regulatory mechanism of mannitol metabolism it is of interest to find the enzyme that initiates the breakdown of mannitol ...
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Molecular analysis of the GlcNac‐1‐phosphotransferase
Journal of Inherited Metabolic Disease, 2008SummaryModification of the carbohydrate chains of soluble lysosomal enzymes with mannose 6‐phosphate residues is a prerequisite for their mannose 6‐phosphate receptor‐dependent transport to lysosomes. GlcNac‐1‐phosphotransferase localized in the Golgi apparatus represents a hexameric α2β2γ2 subunit complex and plays a key role in the formation of the ...
Thomas Braulke +2 more
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Coupled spectrofluorometric assay for aminoglycoside phosphotransferases
Analytical Biochemistry, 1988In order to make accurate kinetic measurements for the substrates of aminoglycoside (AG) phosphotransferases (APHs), we have developed an assay which overcomes many of the limitations of currently used assays. We have adapted the coupled spectrophotometric assay (P. R. Goldman and D. B. Northrop (1976) Biochem. Biophys. Res. Commun.
James P. Greer +2 more
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[58] Clindamycin phosphotransferase
1975Publisher Summary This chapter discusses the assay and purification procedure of clindamycin phosphotransferase. To determine the amounts of clindamycin in reaction mixtures, a standard assay is employed with Sarcina lutea ATCC 9341 as the assay organism.
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Ethanolamine and Choline Phosphotransferases of Chicken Brain
1978Phospholipids are found almost exclusively in cellular membranes. Ethanolamine phosphoglycerides and choline phosphoglycerides are quantitatively the most important. They are in a constant state of metabolic flux with the rates of their synthesis and degradation often varying with the physiological activity of the tissues.
Lloyd A. Horrocks +5 more
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The Bacterial Phosphoenolpyruvate Phosphotransferase System
1976Translocation of certain carbohydrates (monosaccharides and disaccharides) across the cytoplasmic membranes of bacteria can occur by two major processes. These processes are distinguished by the nature of the primary energy source involved as well as the actual mechanism by which the translocation proceeds.
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[24] ATP: Inosamine phosphotransferase(s)
1975Publisher Summary This chapter presents the assay procedure and properties of inosaminc phosphotransferase. In assay, labeled phosphorylated product is separated from labeled substrate by paper chromatography or high-voltage paper electrophoresis and counted.
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