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Integrated Photoacoustic and Fluorescence Confocal Microscopy [PDF]

IEEE Transactions on Biomedical Engineering, 2010
We have developed a dual-modality imaging system by integrating optical-resolution photoacoustic microscopy and fluorescence confocal microscopy to provide optical absorption and fluorescence contrasts simultaneously. By sharing the same laser source and objective lens, intrinsically registered photoacoustic and fluorescence images are acquired in a ...
Chulhong Kim, Lihong V. Wang, Song Hu
exaly   +8 more sources

Photoacoustic Microscopy [PDF]

Laser and Photonics Reviews, 2013
AbstractPhotoacoustic microscopy (PAM) is a hybridin vivoimaging technique that acoustically detects optical contrast via the photoacoustic effect. Unlike pure optical microscopic techniques, PAM takes advantage of the weak acoustic scattering in tissue and thus breaks through the optical diffusion limit (∼1 mm in soft tissue).
Lihong V. Wang, Junjie Yao
exaly   +5 more sources

Sensitivity of Photoacoustic Microscopy

Photoacoustics, 2014
Building on its high spatial resolution, deep penetration depth and excellent image contrast, 3D photoacoustic microscopy (PAM) has grown tremendously since its first publication in 2005. Integrating optical excitation and acoustic detection, PAM has broken through both the optical diffusion and optical diffraction limits.
Lihong V. Wang, Junjie Yao
exaly   +4 more sources

Shear-Force Photoacoustic Microscopy: Toward Super-Resolution Near-Field Imaging

Laser and Photonics Reviews, 2022
© 2022 Wiley-VCH GmbH.Optical-resolution photoacoustic microscopy (OR-PAM) enables both high-resolution and high-contrast imaging of optical chromophores ranging from biological tissues to inorganic samples.
Chulhong Kim, Junsuk Rho
exaly   +2 more sources

Non-Interferometric Photoacoustic Remote Sensing Microscopy

Light: Science and Applications, 2017
Elasto-optical refractive index modulation due to photoacoustic initial pressure transients produced significant reflection of a probe beam when the absorbing interface had an appreciable refractive index difference.
Roger J Zemp, Kevan Bell, Wei Shi
exaly   +2 more sources

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Differential photoacoustic microscopy technique

Optics Letters, 2013
Photoacoustic microscopy (PAM), whose image quality largely depends on the optical absorption of samples, provides endogenous information for structural and functional imaging. However, PAM technology in general can not provide edge enhancement imaging for absorbing objects.
Hongchun, Tang, Zhilie, Tang, Yongbo, Wu, Qiuxia, Cai, Liru, Wu, Yan, Chi   +5 more
openaire   +2 more sources

Fast wavelet-based photoacoustic microscopy

Journal of the Optical Society of America A, 2021
A novel photoacoustic microscopy (PAM) structure, based on Haar wavelet patterns, is proposed in this paper. Its main goal is to mitigate the PAM imaging resolution and thus the time of its sampling process without compromising the image quality. Owing to the intrinsic nature of wavelet transform, this structure collects spatial and
Hassan, Abbasi, Seyed Masood, Mostafavi, Zahra, Kavehvash   +2 more
openaire   +2 more sources

Translational Photoacoustic Microscopy

2015
© 2015 Springer Singapore.
Zhou, Yong, Wang, Lihong V.
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Photoacoustic expansion microscopy of melanosomes

Optics Letters
Optical resolution photoacoustic microscopy (OR-PAM) is a hybrid imaging method for visualizing organelles due to the high spatial resolution and abundant optical contrast. Usually, OR-PAM employs high numerical aperture (NA) objectives and high-frequency ultrasonic detectors to resolve three-dimensional (3D) microstructures of cells.
Tingting Li, Xinrui Gong, Heng Guo, Lei Xi   +3 more
openaire   +2 more sources

Ultracompact high-resolution photoacoustic microscopy

Optics Letters, 2018
Optical resolution photoacoustic microscopy (ORPAM), benefiting from rich optical contrast, scalable acoustic resolution, and deep penetration depth, is of great importance for the fields of biology and medicine. However, limited by the size and performance of reported optical/acoustic scanners, existing portable/handheld ORPAMs are bulky and heavy ...
Qian Chen, Heng Guo, Tian Jin, Weizhi Qi, Huikai Xie, Lei Xi   +5 more
openaire   +2 more sources