Angewandte Chemie, EarlyView.In this review, we discuss how biomolecular condensates can inhibit amyloid aggregation in their interior, while still facilitating fibril formation at the interface between the dense and dilute phases, where molecular and mesoscale properties are likely optimal to promote protein aggregation.
Marcell Papp +3 more
wiley +2 more sources
Photobleaching-based autofluorescence mapping reveals distinct patterns in sporadic basal cell carcinoma, nevoid basal cell carcinoma syndrome-associated basal cell carcinoma and squamous cell carcinoma. [PDF]
Front Med (Lausanne)Plorina EV +7 more
europepmc +1 more source
Advanced Intelligent Discovery, EarlyView.Sequential multicolor fluorescence imaging in dynamic microsystems is constrained by acquisition speed and excitation dose. This study introduces a real‐time framework to reconstruct spectrally separated channels from reduced cross‐channel acquisitions (frames containing mixed spectral contributions).
Juan J. Huaroto +3 more
wiley +1 more source
Light‐Controlled Modulation of 15‐Lipoxygenase‐1 Regulates Intestinal Inflammatory Signaling
Angewandte Chemie, EarlyView.The developed target‐guided strategy enables the rational design of diazo‐based photoswitchable inhibitors of 15‐lipoxygenase‐1. Distinct molecular architectures program opposite light‐dependent activity, allowing on‐demand control of enzyme inhibition.
Anastasia Louka +10 more
wiley +2 more sources
A Standardized Prism-Based TIRF Platform for Quantitative Single-Molecule Fluorescence Studies of Biomolecular Dynamics. [PDF]
Biosensors (Basel)Patra A +4 more
europepmc +1 more source
Cell Segmentation Beyond 2D—A Review of the State‐of‐the‐Art
Advanced Intelligent Discovery, EarlyView.Cell segmentation underpins many biological image analysis tasks, yet most deep learning methods remain limited to 2D despite the inherently 3D nature of cellular processes. This review surveys segmentation approaches beyond 2D, comparing 2.5D and fully 3D methods, analyzing 31 models and 32 volumetric datasets, and introducing a unified reference ...
Fabian Schmeisser +6 more
wiley +1 more source
Novel Non‐Cytotoxic Acylphosphinates and Acylphosphine Oxides Photoinitiators
Angewandte Chemie, EarlyView.Novel acylphosphine oxide (APO) and acylphosphinate photoinitiators based on DPO and DOPO were designed to investigate structure–property relationships including photochemical performance, polymerization efficiency and cytotoxicity. While DPO derivatives showed excellent reactivity, DOPO‐based systems also displayed good efficiency and significantly ...
Jiansong Yin +13 more
wiley +2 more sources
NanorulerQA: quantitative quality analysis of dual-color DNA nanorulers via single-molecule photobleaching step counting and spatio-temporal colocalization. [PDF]
Biomed Opt ExpressWu J +8 more
europepmc +1 more source
Thermometric Based‐Microswimmers with Chemical and Optical Engines
Advanced Intelligent Systems, EarlyView.Temperature sensing at small scales is typically performed using passive luminescent particles. Here, an alternative approach is demonstrated by integrating upconversion thermometry into self‐propelled microswimmers powered by chemical fuels or light. This strategy offers a step toward dynamic thermal sensing at the microscale, relevant to both lab‐on ...
João M. Gonçalves, Katherine Villa
wiley +1 more source
Perilipin‐2‐Anchored Lipid Droplet‐Targeting Phototheranostics
Angewandte Chemie, EarlyView.This study reports two NIR theranostic dyes, C‐BDP‐OH and C‐BDP‐OMe. C‐BDP‐OMe targets lipid droplets via a dual‐anchoring mechanism with PLIN2, enabling wash‐free tumor imaging. With 62.6% photothermal conversion efficiency, it achieves tumor ablation by downregulating PLIN2, disrupting LD‐mitochondria crosstalk, and triggering apoptosis and ...
Lipeng Zhang +9 more
wiley +2 more sources