Results 41 to 50 of about 16,087 (217)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Mediating Islamic State| Toward a Protostate Media System:The Role of ISIS’s Content

open access: yesInternational Journal of Communication, 2020
Militant groups introduce a layer of complexity to existing theories of media systems. Operating as a protostate, ISIS defies the common understandings of Hallin and Mancini’s four dimensions of national media systems by blurring the lines among the ...
Kareem El Damanhoury
doaj  

Lost in Reintegration?

open access: yesCeROArt : Conservation, Exposition, Restauration d'Objets d'Art, 2015
This article describes different approaches to the issue of image reintegration and filling in the areas of loss in photographs and photographic postcards.
Magdalena Grenda
doaj   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

open access: yesFEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia   +3 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Photographic Images of Refugee Spatial Encounters: Pedagogy of Displacement

open access: yesQualitative Research in Education, 2013
This paper examines my effort to document the experiences of a Bhutanese refugee community in a mid-western city of the United States. In particular, the essay looks at housing experiences the community encountered and my efforts to translate the events ...
Binaya Subedi
doaj  

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

open access: yesFEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey   +4 more
wiley   +1 more source

Transforming and enhancing metadata for enduser discovery: a case study

open access: yesJLIS.it, 2014
This paper describes the process developed by Binghamton University Libraries to extract embedded metadata from digital photographs and transform it into descriptive Dublin Core metadata for use in the Libraries’ digital preservation system.
Edward M. Corrado, Rachel Jaffe
doaj   +1 more source

Evaluating the effect of γ‐oryzanol on MASLD pathology using a medaka fish model

open access: yesFEBS Open Bio, EarlyView.
This study explores a liver disease called MASLD, which is increasing worldwide and can lead to serious damage. Researchers used medaka fish instead of rodents to test a food compound, γ‐oryzanol. Fish fed this compound had less liver fat and healthier gut bacteria.
Yukako Ito   +7 more
wiley   +1 more source

Imaging Analysis and Digital Restoration of the Holy Face of Manoppello—Part II

open access: yesHeritage, 2018
In this second work on the Holy Face of Manoppello, known also as the Veil, we have performed a spectral analysis of the transmitted image. Its thin linen threads are translucent, presumably because starched, allowing light to pass through them.
Liberato De Caro   +2 more
doaj   +1 more source

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