Results 61 to 70 of about 244,562 (235)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

PROPRIEDADES FÍSICO-MECÂNICAS DE PAINÉIS AGLOMERADOS COM Bambusa tuldoides E Pinus taeda

Ciência Florestal, 2015
This study investigated the physical and mechanical properties of particleboards formed of bamboo (Bambusa tuldoides) and pine (Pinus taeda) and combinations between them.
Weslley Wilker Corrêa Morais, Clovis Roberto Haselein, Felipe Susin, Magnos Alan Vivian, Janice Bittencourt Facco Morais   +4 more
doaj  

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Chromated copper arsenate treatment on paricá wood [PDF]

Ciência Rural
: This study investigated the influence of a commercial treatment used for Eucalyptus sp. fenceposts with chromated copper arsenate (CCA) on paricá (Schizolobium parahyba var. amazonicum) round wood.
João Miguel Santos Dias, Niara Porto de Carvalho, Maria Gabriela Sales da Silva, Vinícius de Sousa Lima, Itallo Gabriel Oliveira Locatelli, Diana Neres dos Santos, David Januário de Sousa, Marco Antonio Siviero, Sabrina Benmuyal Vieira, Agust Sales, Rita Dione Araújo Cunha, Sandro Fábio César   +11 more
doaj   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Predicting and Comparing the Subjective Health Experience of Older Cancer Survivors and Non‐Cancer Survivors: A Modeling Approach

Aging and Cancer, EarlyView.
This study underscores the significant influence of frailty and vitality on the subjective health experience of older cancer survivors with acceptance and control emerging as salient mediators. These findings affirm the conceptual and empirical robustness of the model highlighting its potential utility in shaping future interventions for older cancer ...
Damien S. E. Broekharst, Sjaak Bloem, Eline J. Mertens, Nathascha Hanzen, Michel van Agthoven   +4 more
wiley   +1 more source

Determination of the Optimal Polypropylene Fiber Addition to the Dense Bituminous Mixtures by the Aid of Mechanical and Optical Means

The Baltic Journal of Road and Bridge Engineering, 2012
Polypropylene fibers are completely native modifiers and they do not have any dependence on abroad in case of technology. When viewed from this point, the modification of bituminous binders with polypropylene fibers is a very important step for our ...
Serkan Tapkin, Şenol Özcan
doaj   +1 more source

Visual Recovery Reflects Cortical MeCP2 Sensitivity in Rett Syndrome

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Rett syndrome (RTT) is a devastating neurodevelopmental disorder with developmental regression affecting motor, sensory, and cognitive functions. Sensory disruptions contribute to the complex behavioral and cognitive difficulties and represent an important target for therapeutic interventions.
Alex Joseph Simon, Nathalie Picard, Valeria d'Andrea, Enchi Chang, Joseph Leffler, Eleonora Centofante, Matthew Taylor, Francesca Bardi, Francesca Cavicchiolo, Takao K. Hensch, Stefano Panzeri, Chinfei Chen, Michela Fagiolini   +12 more
wiley   +1 more source

Parameter determination of Johnson–Holmquist–Cook constitutive model and calibration for Indiana Limestone

Geomechanics and Geophysics for Geo-Energy and Geo-Resources
In this study, a comprehensive parameter determination procedure for the Johnson–Holmquist–Cook (JHC) constitutive model is introduced, including calibration and validation processes for Indiana Limestone rocks.
Mahdi Heydari, Ebrahim Farrokh, Seyed Hasan Khoshrou   +2 more
doaj   +1 more source