Results 71 to 80 of about 209,650 (263)

Single‐cell DNA methylation profiling: Technologies, computation, and applications in precision oncology

Molecular Oncology, EarlyView.
Single‐cell DNA methylation (scDNAme) profiling maps epimutational clonal evolution, revealing mechanisms of malignancy and therapeutic resistance across diverse cancer types. By providing a high‐resolution landscape of intratumoral heterogeneity, these technologies empower precise patient stratification, guide the development of enhanced ...
Ik Soo Kim
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Morphological, physical attributes and match performance in young handball players

E-balonmano.com: Revista de Ciencias del Deporte, 2021
The aim of this study was threefold: 1) to compare the morphological and physical attributes of the young players divided into two different maturational groups; 2) to  analyze the association between the morphological and physical attributes and the ...
Renato Resina de Oliveira Campos, Anna Volossovitch, António Paulo Pereira Ferreira   +2 more
doaj  

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Comprehensive Quality Analysis of Common Vetch (Vicia sativa L.) Varieties Using Image Processing Techniques and Artificial Intelligence

Agriculture
Common vetch (Vicia sativa L.) is a cool-season annual legume cultivated for grain and forage, valued for its high nutrient content, broad edaphoclimatic adaptability, and suitability for crop rotations.
Necati Çetin, Onur Okumuş, Satı Uzun, Mahmut Kaplan, Ahmad Jahanbakhshi, Gniewko Niedbała   +5 more
doaj   +1 more source

Ozonation improves physical attributes in domestic sewage effluent

Revista Ambiente & Água, 2019
Root Zone Wastewater Treatment (RZWT) is an option of natural wastewater treatment widely studied throughout the world, being a technology based on physical, chemical and biological processes.
Osli Barreto Camilo Júnior, Delvio Sandri, Ernandes Rodrigues de Alencar, Lucas Ferraz Hebling   +3 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Application of treated domestic sewage effluent on the quality indicators of an Oxisol cultivated with castor bean

Revista Ceres
In semi-arid regions of developing countries worldwide it is necessary to develop low cost technology methods to acquire new water supplies. The objective was to evaluate the changes in the attributes of soil quality irrigated with treated sewage ...
Adailton Conceição dos Santos, Cácio Luiz Boechat, Paloma Cunha Saraiva, Adriana Miranda de Santana Arauco, Felizardo Adenilson Rocha, Maria de Fátima da Silva Pinto Peixoto   +5 more
doaj   +1 more source