Results 131 to 140 of about 5,816,150 (303)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Corrigendum to "Long-Term Potable Effects of Alkalescent Mineral Water on Intestinal Microbiota Shift and Physical Conditioning". [PDF]

Evid Based Complement Alternat Med, 2020
Yahiro T, Hara T, Matsumoto T, Ikebe E, Fife-Koshinomi N, Xu Z, Hiratsuka T, Iha H, Inomata M.   +8 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Acute effects of movement-specific warm-up on force production and neuromuscular activation during maximal isometric squat in resistance-trained men: a pilot study

European Journal of Translational Myology
Warm-up is a fundamental preparatory phase for optimizing performance, yet its acute effects may depend on the specificity of the protocol to the target task.
Viktor Oliva, Julián E. Smoliga, Matej Tóth, Gabriel Buzgó   +3 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Amplitude of Biceps Femoris Activation and Triaxial Acceleration in a 50-Meter Test in Sprinters: Pilot Study

Applied Sciences
Objective: To describe the relationship between bilateral electrical activity in the biceps femoris and the variation of triaxial acceleration in three 50 m sprints.
Julio Martín-Ruiz, Ignacio Tamarit-Grancha, Clara Gallego-Cerveró, Alberto Pardo-Ibáñez, Carlos Cordente-Martínez, Laura Ruiz-Sanchis   +5 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Relating Question Type to Panel Conditioning: A Comparison between Trained and Fresh Respondents [PDF]

Panel conditioning arises if respondents are influenced by participation in previous surveys, such that their answers differ significantly from the answers of individuals who are interviewed for the first time.
Toepoel, V., Das, J.W.M., Soest, A.H.O. van   +2 more
core  

Inter-Limb Upper-Limb Strength Asymmetry and Rifle Shooting Performance Across Prone, Kneeling and Standing Positions in Elite Rifle Athletes

Applied Sciences
Background: Rifle shooting performance depends on precise postural control, rifle stability, and coordinated upper-limb force production. Although previous studies have examined postural balance and aiming stability in rifle shooters, the role of upper ...
Yincheng Wei, Shibo Ling, Shengyu Cui, Shiwei Song, Andrew Soundy   +4 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source