Results 111 to 120 of about 103,184 (265)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Acute effects of movement-specific warm-up on force production and neuromuscular activation during maximal isometric squat in resistance-trained men: a pilot study

European Journal of Translational Myology
Warm-up is a fundamental preparatory phase for optimizing performance, yet its acute effects may depend on the specificity of the protocol to the target task.
Viktor Oliva, Julián E. Smoliga, Matej Tóth, Gabriel Buzgó   +3 more
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Amplitude of Biceps Femoris Activation and Triaxial Acceleration in a 50-Meter Test in Sprinters: Pilot Study

Applied Sciences
Objective: To describe the relationship between bilateral electrical activity in the biceps femoris and the variation of triaxial acceleration in three 50 m sprints.
Julio Martín-Ruiz, Ignacio Tamarit-Grancha, Clara Gallego-Cerveró, Alberto Pardo-Ibáñez, Carlos Cordente-Martínez, Laura Ruiz-Sanchis   +5 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Inter-Limb Upper-Limb Strength Asymmetry and Rifle Shooting Performance Across Prone, Kneeling and Standing Positions in Elite Rifle Athletes

Applied Sciences
Background: Rifle shooting performance depends on precise postural control, rifle stability, and coordinated upper-limb force production. Although previous studies have examined postural balance and aiming stability in rifle shooters, the role of upper ...
Yincheng Wei, Shibo Ling, Shengyu Cui, Shiwei Song, Andrew Soundy   +4 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Effect of Linear Sprints and Change-of-Direction Training Versus Small-Sided Soccer Games on Physical Performance in Highly Trained Young Female Soccer Players: A Randomized Cross-Over Study

Sports
Background: This study aimed to compare the effects of linear sprint training with changes of direction (LSCD) versus small-sided games (SSSG) on physical performance, agility, and soccer-specific skills in young elite female players.
Abdelwahid Aboulfaraj, Fatiha Laziri, Salah Eddine Haddou, Salah Lahlou, Mohamed Aghrouch, Ali Belamjahad, Juan Del Coso, Luca Paolo Ardigò, Hassane Zouhal   +8 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

PHYSICAL FORCE AS AN ADMISSIBILITY CONDITION IN PHYSICS

This paper isolates physical force as a candidate admissibility condition for physical reality and tests it under a strict elimination-by-necessity framework. Force is examined solely as a physically enforcing mechanism acting in spacetime, independent of mathematical formalisms, descriptions, or interpretations.
openaire   +2 more sources