Results 101 to 110 of about 104,124 (265)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Ischemic Preconditioning (IPC) Enhances the Accuracy and Stability of Proprioception

Applied Sciences
This study aimed to investigate the differences in proprioceptive changes at different time points (Pre vs. Post vs. 90 min vs. 24 h) before and after ischemic preconditioning.
Junqi Wu, Peng Zhang, Yecheng Zhang, Yuying Su, Yu Shi, Chunlei Li   +5 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Absence and the Physical Conditions of Work [PDF]

Occupational and Environmental Medicine, 1957
R D, SHEPHERD, J, WALKER
openaire   +2 more sources

Response of skin temperature, blood ammonia and lactate during incremental exercise until exhaustion in elite athletes

Scientific Reports
The study aimed to evaluate the lower limb skin temperature (Tsk) and blood concentrations of lactate (LA) and ammonia (NH3) during exercise and recovery.
Paweł Korman, Krzysztof Kusy, Anna Straburzyńska-Lupa, Adam Kantanista, Manuel Sillero Quintana, Jacek Zieliński   +5 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Corrigendum to "Long-Term Potable Effects of Alkalescent Mineral Water on Intestinal Microbiota Shift and Physical Conditioning". [PDF]

Evid Based Complement Alternat Med, 2020
Yahiro T, Hara T, Matsumoto T, Ikebe E, Fife-Koshinomi N, Xu Z, Hiratsuka T, Iha H, Inomata M.   +8 more
europepmc   +1 more source

Acute effects of movement-specific warm-up on force production and neuromuscular activation during maximal isometric squat in resistance-trained men: a pilot study

European Journal of Translational Myology
Warm-up is a fundamental preparatory phase for optimizing performance, yet its acute effects may depend on the specificity of the protocol to the target task.
Viktor Oliva, Julián E. Smoliga, Matej Tóth, Gabriel Buzgó   +3 more
doaj   +1 more source