Results 71 to 80 of about 103,012 (264)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Efficacy and moderators of isometric resistance training (IRT) on resting blood pressure among patients with pre- to established hypertension: a multilevel meta-review and regression analysis

BMC Sports Science, Medicine and Rehabilitation
Objective This study aims to evaluate the efficacy of isometric resistance training (IRT) in lowering blood pressure through meta-analyses, specifically systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean arterial pressure (MAP), in ...
Chengyu Zhou, Sijia Li, Zengyu Zhang, Jiamin Chen, Jian Song, Yongmin Xie, Zhuolin Tang, Aiguo Zhou   +7 more
doaj   +1 more source

Comparative Study of the Sprint Start Biomechanics of Men’s 100 m Athletes of Different Levels

Applied Sciences
Background: This study sought to investigate the kinematic and kinetic differences in sprint start between high-level and medium-level sprinters. Methods: Twenty male sprinters were dichotomized according to their personal 100 m performance.
Zhao Li, Yan Peng, Qing Li
doaj   +1 more source

Propuesta de intervención educativa para la enseñanza del aparato locomotor en el Grado de Técnico Superior en Acondicionamiento Físico [PDF]

Ikastorratza.e-Revista de Didáctica
This paper presents an innovative intervention proposal to teach the unit "Biomechanical: structures implicated in movement" in the Higher Technician Grade in Physical Conditioning Certificate. The main goal of this proposal is to provide students with a
Carolina Ansó López, María Casas Jericó   +1 more
doaj   +1 more source

Physical Conditioning Strategies for the Prevention of Concussion in Sport: a Scoping Review. [PDF]

Sports Med Open, 2021
Garnett D, Patricios J, Cobbing S.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Exercise and tissue-resident memory T cells: from circulating numbers to spatial immune remodeling

Frontiers in Immunology
Tissue-resident memory T (TRM) cells have become a paradigm shift in the field of immunology and have changed our view of local immune surveillance at barrier surfaces. In contrast to circulating memory T cells, TRM cells are fixed in non-lymphoid organs
Enli Xie, Yushan He, Tainan Cao, Changchun Li, Zhiming Wang   +4 more
doaj   +1 more source

Soldier Load Carriage, Injuries, Rehabilitation and Physical Conditioning: An International Approach. [PDF]

Int J Environ Res Public Health, 2021
Orr R, Pope R, Lopes TJA, Leyk D, Blacker S, Bustillo-Aguirre BS, Knapik JJ.   +6 more
europepmc   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source