Results 91 to 100 of about 21,771,545 (251)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Mangroves as Coastal Protection for Restoring Low-Energy Waterfront Property

Journal of Marine Science and Engineering
Mangroves offer vital ecological advantages including air and water filtration, coastal and estuarine habitat provision, sediment stabilization, and wave energy dissipation.
Robert J. Weaver, Abigail L. Stehno
doaj   +1 more source

Numeric Simulation of Heat Transfer from a Single Round Tube Shielded with Wire Mesh [PDF]

Problems of the Regional Energetics, 2015
This paper presents the results of development and investigation of heat transfer at transverse flow of round tube with wire screen using the software ANSYS Fluent 3D-model.
Dymo B.V., Pastuhov S.Y., Chegryntsev V.F., Anastasenko S.N.   +3 more
doaj  

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Physical Model Study on Wave Attenuation Effects by Forests on Horizontal and Gentle Slope Beaches

Renmin Zhujiang
This study systematically investigated the variation patterns of key hydrodynamic parameters, including wave height, wave pressure, plant stem force, and three-dimensional (3D) flow velocity through large-scale physical model experiments of wave flumes ...
ZHANG Zhilin, HUANG Bensheng, LIU Da, HONG Changhong, HU Tao   +4 more
doaj  

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

A Comparison and Analysis of Virtual and Physical Testing Using a Simple Free-Falling Problem

Engineering Proceedings
This study explores a simple free-falling problem, focusing on the determination of the coefficient of restitution for a steel ball through virtual and physical experiment evaluation. Different effects influencing the dynamic behavior and accuracy of the
Georgi Todorov, Konstantin Kamberov, Konstantin Dimitrov   +2 more
doaj   +1 more source

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source

The Brisbane Model: considering a unique experiment

, 1995
Brisbane's experiment with large-scale local government is often extolled as the superior model for sub-national public administration. This article weighs the Brisbane model's advantages against disadvantages including complexity, community ...
Stockwell, Stephen
core   +1 more source

The C‐terminal truncated splicing variant of NK1R negatively modulates substance P‐stimulated NK1R signaling

FEBS Open Bio, EarlyView.
The neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Lan Phuong Nguyen, Duc Trung Nguyen, Minyoung Cho, Jihun Kim, Soyeon In, Thai Uy Nguyen, Sunghoon Hurh, Beom Jin Park, Jong‐Ik Hwang   +8 more
wiley   +1 more source